The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin.

Journal Article (Journal Article)

BACKGROUND: Vascular smooth muscle cell (VSMC) migration is an important process in many vascular disorders. Nicotine, thrombospondin-1 (TSP-1) and fibronectin (Fn) separately induce VSMC migration. The hypothesis of this study was that nicotine treatment of vascular cells would augment TSP-1-induced and Fn-induced VSMC migration. METHODS: VSMCs or endothelial cells (ECs) were treated with serum-free medium or nicotine. Migration of VSMCs was assessed using a modified Boyden chemotaxis chamber to serum-free medium, TSP-1, Fn, EC basal medium, and conditioned EC medium or nicotine-treated conditioned EC medium alone or with supplemented TSP-1 or Fn. RESULTS: Nicotine treatment increased VSMC chemotaxis to serum-free medium, but TSP-1 or Fn had no further effect on chemotaxis. Conditioned EC and nicotine-treated conditioned EC enhanced VSMC chemotaxis, which was further augmented by Fn supplementation. CONCLUSIONS: Nicotine-stimulated EC derived factors induce VSMC migration, which is augmented by the addition of Fn.

Full Text

Duke Authors

Cited Authors

  • Stein, JJ; Seymour, KA; Maier, KG; Gahtan, V

Published Date

  • November 2011

Published In

Volume / Issue

  • 202 / 5

Start / End Page

  • 545 - 548

PubMed ID

  • 21944292

Electronic International Standard Serial Number (EISSN)

  • 1879-1883

Digital Object Identifier (DOI)

  • 10.1016/j.amjsurg.2011.06.016


  • eng

Conference Location

  • United States