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The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin.

Publication ,  Journal Article
Stein, JJ; Seymour, KA; Maier, KG; Gahtan, V
Published in: Am J Surg
November 2011

BACKGROUND: Vascular smooth muscle cell (VSMC) migration is an important process in many vascular disorders. Nicotine, thrombospondin-1 (TSP-1) and fibronectin (Fn) separately induce VSMC migration. The hypothesis of this study was that nicotine treatment of vascular cells would augment TSP-1-induced and Fn-induced VSMC migration. METHODS: VSMCs or endothelial cells (ECs) were treated with serum-free medium or nicotine. Migration of VSMCs was assessed using a modified Boyden chemotaxis chamber to serum-free medium, TSP-1, Fn, EC basal medium, and conditioned EC medium or nicotine-treated conditioned EC medium alone or with supplemented TSP-1 or Fn. RESULTS: Nicotine treatment increased VSMC chemotaxis to serum-free medium, but TSP-1 or Fn had no further effect on chemotaxis. Conditioned EC and nicotine-treated conditioned EC enhanced VSMC chemotaxis, which was further augmented by Fn supplementation. CONCLUSIONS: Nicotine-stimulated EC derived factors induce VSMC migration, which is augmented by the addition of Fn.

Duke Scholars

Published In

Am J Surg

DOI

EISSN

1879-1883

Publication Date

November 2011

Volume

202

Issue

5

Start / End Page

545 / 548

Location

United States

Related Subject Headings

  • Thrombospondin 1
  • Surgery
  • Nicotine
  • Myocytes, Smooth Muscle
  • Muscle, Smooth, Vascular
  • Humans
  • Ganglionic Stimulants
  • Fibronectins
  • Endothelial Cells
  • Chemotaxis
 

Citation

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Stein, J. J., Seymour, K. A., Maier, K. G., & Gahtan, V. (2011). The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin. Am J Surg, 202(5), 545–548. https://doi.org/10.1016/j.amjsurg.2011.06.016
Stein, Jeffrey J., Keri A. Seymour, Kristopher G. Maier, and Vivian Gahtan. “The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin.Am J Surg 202, no. 5 (November 2011): 545–48. https://doi.org/10.1016/j.amjsurg.2011.06.016.
Stein JJ, Seymour KA, Maier KG, Gahtan V. The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin. Am J Surg. 2011 Nov;202(5):545–8.
Stein, Jeffrey J., et al. “The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin.Am J Surg, vol. 202, no. 5, Nov. 2011, pp. 545–48. Pubmed, doi:10.1016/j.amjsurg.2011.06.016.
Stein JJ, Seymour KA, Maier KG, Gahtan V. The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin. Am J Surg. 2011 Nov;202(5):545–548.
Journal cover image

Published In

Am J Surg

DOI

EISSN

1879-1883

Publication Date

November 2011

Volume

202

Issue

5

Start / End Page

545 / 548

Location

United States

Related Subject Headings

  • Thrombospondin 1
  • Surgery
  • Nicotine
  • Myocytes, Smooth Muscle
  • Muscle, Smooth, Vascular
  • Humans
  • Ganglionic Stimulants
  • Fibronectins
  • Endothelial Cells
  • Chemotaxis