The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin.
BACKGROUND: Vascular smooth muscle cell (VSMC) migration is an important process in many vascular disorders. Nicotine, thrombospondin-1 (TSP-1) and fibronectin (Fn) separately induce VSMC migration. The hypothesis of this study was that nicotine treatment of vascular cells would augment TSP-1-induced and Fn-induced VSMC migration. METHODS: VSMCs or endothelial cells (ECs) were treated with serum-free medium or nicotine. Migration of VSMCs was assessed using a modified Boyden chemotaxis chamber to serum-free medium, TSP-1, Fn, EC basal medium, and conditioned EC medium or nicotine-treated conditioned EC medium alone or with supplemented TSP-1 or Fn. RESULTS: Nicotine treatment increased VSMC chemotaxis to serum-free medium, but TSP-1 or Fn had no further effect on chemotaxis. Conditioned EC and nicotine-treated conditioned EC enhanced VSMC chemotaxis, which was further augmented by Fn supplementation. CONCLUSIONS: Nicotine-stimulated EC derived factors induce VSMC migration, which is augmented by the addition of Fn.
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- Thrombospondin 1
- Surgery
- Nicotine
- Myocytes, Smooth Muscle
- Muscle, Smooth, Vascular
- Humans
- Ganglionic Stimulants
- Fibronectins
- Endothelial Cells
- Chemotaxis
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Thrombospondin 1
- Surgery
- Nicotine
- Myocytes, Smooth Muscle
- Muscle, Smooth, Vascular
- Humans
- Ganglionic Stimulants
- Fibronectins
- Endothelial Cells
- Chemotaxis