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Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA.

Publication ,  Journal Article
Ramaswamy, P; Powers, JG; Bhawan, J; Polyak, I; Gilchrest, BA
Published in: Dermatol Surg
September 2014

BACKGROUND: Photodynamic therapy (PDT) using aminolevulinic acid (ALA) with blue light or red light is effective for treating actinic keratoses (AKs). However, immunosuppression follows red light PDT, raising the spectre of skin cancer promotion in treated skin. OBJECTIVE: To determine whether broad-area short incubation (BASI)-ALA-PDT using blue light immunosuppression immunosuppresses treated skin. METHODS: Patients were evaluated clinically and by standardized facial biopsies of non-AK skin before, 24 hours and 1 month after customary blue light BASI-ALA-PDT. All biopsies were stained for markers of epidermal atypia and Langerhans cells (LCs); and at 24 hours to detect oxidative DNA damage. RESULTS: Patients had an 81% reduction in AKs and slight improvement in clinical and histologic signs of photoaging after 1 month. The biopsied chronically photodamaged skin without clinically detectable AKs showed no effect of PDT on the LC number, distribution, or morphology; and no oxidative DNA damage, in contrast to the changes reported after customary red light PDT. CONCLUSION: Customary blue light BASI-ALA-PDT does not affect the LC number or produce oxidative DNA damage, the sequelae of red light PDT responsible for immunosuppression in treated skin.

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Published In

Dermatol Surg

DOI

EISSN

1524-4725

Publication Date

September 2014

Volume

40

Issue

9

Start / End Page

979 / 987

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Skin Aging
  • Photosensitizing Agents
  • Photochemotherapy
  • Oxidative Stress
  • Middle Aged
  • Male
  • Langerhans Cells
  • Ki-67 Antigen
  • Keratosis, Actinic
 

Citation

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Ramaswamy, P., Powers, J. G., Bhawan, J., Polyak, I., & Gilchrest, B. A. (2014). Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA. Dermatol Surg, 40(9), 979–987. https://doi.org/10.1097/01.DSS.0000452624.01889.8a
Ramaswamy, Preethi, Jennifer G. Powers, Jag Bhawan, Ildiko Polyak, and Barbara A. Gilchrest. “Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA.Dermatol Surg 40, no. 9 (September 2014): 979–87. https://doi.org/10.1097/01.DSS.0000452624.01889.8a.
Ramaswamy P, Powers JG, Bhawan J, Polyak I, Gilchrest BA. Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA. Dermatol Surg. 2014 Sep;40(9):979–87.
Ramaswamy, Preethi, et al. “Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA.Dermatol Surg, vol. 40, no. 9, Sept. 2014, pp. 979–87. Pubmed, doi:10.1097/01.DSS.0000452624.01889.8a.
Ramaswamy P, Powers JG, Bhawan J, Polyak I, Gilchrest BA. Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA. Dermatol Surg. 2014 Sep;40(9):979–987.
Journal cover image

Published In

Dermatol Surg

DOI

EISSN

1524-4725

Publication Date

September 2014

Volume

40

Issue

9

Start / End Page

979 / 987

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Skin Aging
  • Photosensitizing Agents
  • Photochemotherapy
  • Oxidative Stress
  • Middle Aged
  • Male
  • Langerhans Cells
  • Ki-67 Antigen
  • Keratosis, Actinic