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Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine.

Publication ,  Journal Article
Ikeda, TS; Hwang, ES; Coady, MJ; Hirayama, BA; Hediger, MA; Wright, EM
Published in: J Membr Biol
August 1989

The Na+/glucose cotransporter from rabbit intestinal brush border membranes has been cloned, sequenced, and expressed in Xenopus oocytes. Injection of cloned RNA into oocytes increased Na+/sugar cotransport by three orders of magnitude. In this study, we have compared and contrasted the transport properties of this cloned protein expressed in Xenopus oocytes with the native transporter present in rabbit intestinal brush borders. Initial rates of 14C-alpha-methyl-D-glucopyranoside uptake into brush border membrane vesicles and Xenopus oocytes were measured as a function of the external sodium, sugar, and phlorizin concentrations. Sugar uptake into oocytes and brush borders was Na+-dependent (Hill coefficient 1.5 and 1.7), phlorizin inhibitable (Ki 6 and 9 microM), and saturable (alpha-methyl-D-glucopyranoside Km 110 and 570 microM). The sugar specificity was examined by competition experiments, and in both cases the selectivity was D-glucose greater than alpha-methyl-D-glucopyranoside greater than D-galactose greater than 3-O-methyl-D-glucoside. In view of the close similarity between the properties of the cloned protein expressed in oocytes and the native brush border transporter, we conclude that we have cloned the classical Na+/glucose cotransporter.

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Published In

J Membr Biol

DOI

ISSN

0022-2631

Publication Date

August 1989

Volume

110

Issue

1

Start / End Page

87 / 95

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Sodium
  • Rabbits
  • Physiology
  • Phlorhizin
  • Monosaccharide Transport Proteins
  • Intestine, Small
  • Glucose
  • Cloning, Molecular
  • Animals
 

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Ikeda, T. S., Hwang, E. S., Coady, M. J., Hirayama, B. A., Hediger, M. A., & Wright, E. M. (1989). Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine. J Membr Biol, 110(1), 87–95. https://doi.org/10.1007/BF01870995
Ikeda, T. S., E. S. Hwang, M. J. Coady, B. A. Hirayama, M. A. Hediger, and E. M. Wright. “Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine.J Membr Biol 110, no. 1 (August 1989): 87–95. https://doi.org/10.1007/BF01870995.
Ikeda TS, Hwang ES, Coady MJ, Hirayama BA, Hediger MA, Wright EM. Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine. J Membr Biol. 1989 Aug;110(1):87–95.
Ikeda, T. S., et al. “Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine.J Membr Biol, vol. 110, no. 1, Aug. 1989, pp. 87–95. Pubmed, doi:10.1007/BF01870995.
Ikeda TS, Hwang ES, Coady MJ, Hirayama BA, Hediger MA, Wright EM. Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine. J Membr Biol. 1989 Aug;110(1):87–95.
Journal cover image

Published In

J Membr Biol

DOI

ISSN

0022-2631

Publication Date

August 1989

Volume

110

Issue

1

Start / End Page

87 / 95

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Sodium
  • Rabbits
  • Physiology
  • Phlorhizin
  • Monosaccharide Transport Proteins
  • Intestine, Small
  • Glucose
  • Cloning, Molecular
  • Animals