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Distribution of the SGLT1 Na+/glucose cotransporter and mRNA along the crypt-villus axis of rabbit small intestine.

Publication ,  Journal Article
Hwang, ES; Hirayama, BA; Wright, EM
Published in: Biochem Biophys Res Commun
December 31, 1991

The expression of the Na+/glucose cotransporter (SGLT1) mRNA and protein along the crypt-villus axis of the rabbit small intestine was examined using in situ hybridization and immunocytochemical techniques. We detected mRNA in the cells on the villus, but not in the crypts, and the mRNA abundance increased 6-fold from the base to the tip of the villus. SGLT1 protein was restricted to the brush borders of mature enterocytes. We suggest that the high rate of sugar transport across the tips of the villus is due to the transcription of the SGLT1 gene in mature enterocytes, the subsequent translation of SGLT mRNA, and the insertion direct of the functional SGLT1 transporter into the brush border membrane of these cells lining the villus tip.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

December 31, 1991

Volume

181

Issue

3

Start / End Page

1208 / 1217

Location

United States

Related Subject Headings

  • Sulfur Radioisotopes
  • Rabbits
  • RNA, Messenger
  • RNA Probes
  • Nucleic Acid Hybridization
  • Muscle, Smooth
  • Monosaccharide Transport Proteins
  • Male
  • Jejunum
  • Fluorescent Antibody Technique
 

Citation

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Hwang, E. S., Hirayama, B. A., & Wright, E. M. (1991). Distribution of the SGLT1 Na+/glucose cotransporter and mRNA along the crypt-villus axis of rabbit small intestine. Biochem Biophys Res Commun, 181(3), 1208–1217. https://doi.org/10.1016/0006-291x(91)92067-t
Hwang, E. S., B. A. Hirayama, and E. M. Wright. “Distribution of the SGLT1 Na+/glucose cotransporter and mRNA along the crypt-villus axis of rabbit small intestine.Biochem Biophys Res Commun 181, no. 3 (December 31, 1991): 1208–17. https://doi.org/10.1016/0006-291x(91)92067-t.
Hwang ES, Hirayama BA, Wright EM. Distribution of the SGLT1 Na+/glucose cotransporter and mRNA along the crypt-villus axis of rabbit small intestine. Biochem Biophys Res Commun. 1991 Dec 31;181(3):1208–17.
Hwang, E. S., et al. “Distribution of the SGLT1 Na+/glucose cotransporter and mRNA along the crypt-villus axis of rabbit small intestine.Biochem Biophys Res Commun, vol. 181, no. 3, Dec. 1991, pp. 1208–17. Pubmed, doi:10.1016/0006-291x(91)92067-t.
Hwang ES, Hirayama BA, Wright EM. Distribution of the SGLT1 Na+/glucose cotransporter and mRNA along the crypt-villus axis of rabbit small intestine. Biochem Biophys Res Commun. 1991 Dec 31;181(3):1208–1217.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

December 31, 1991

Volume

181

Issue

3

Start / End Page

1208 / 1217

Location

United States

Related Subject Headings

  • Sulfur Radioisotopes
  • Rabbits
  • RNA, Messenger
  • RNA Probes
  • Nucleic Acid Hybridization
  • Muscle, Smooth
  • Monosaccharide Transport Proteins
  • Male
  • Jejunum
  • Fluorescent Antibody Technique