Measurement of angiotensin II: use of two injectors to minimize HPLC shadowing.
Publication
, Journal Article
Naik, GO; Forster, C; Armstrong, PW
Published in: Peptides
1994
We investigated the use of two HPLC injectors, one reserved for standards and the other for blanks or biological samples, to minimize shadowing in the measurement of angiotensin II (ANGII). HPLC carryover of standard ANGII to blank with a one-injector and a two-injector system were 47.0 +/- 5.0 and 2.4 +/- 0.5 fmol/ml, respectively, a 19.6-fold reduction. Measured normal canine left ventricular myocardium ANGII level by the two-injector HPLC-RIA system was 22.3 +/- 2.4 fmol/g, with a signal-to-noise ratio of 11.7, an improved signal-to-noise ratio of 29.3 fold vs. the one injector. This innovation reduced the incidence of false-positive ANGII results, and thus can be applied to other compounds that exhibit HPLC-derived shadowing.
Duke Scholars
Published In
Peptides
DOI
ISSN
0196-9781
Publication Date
1994
Volume
15
Issue
7
Start / End Page
1255 / 1259
Location
United States
Related Subject Headings
- Reference Standards
- Myocardium
- Male
- Dogs
- Chromatography, High Pressure Liquid
- Animals
- Angiotensins
- Angiotensin II
Citation
APA
Chicago
ICMJE
MLA
NLM
Naik, G. O., Forster, C., & Armstrong, P. W. (1994). Measurement of angiotensin II: use of two injectors to minimize HPLC shadowing. Peptides, 15(7), 1255–1259. https://doi.org/10.1016/0196-9781(94)90150-3
Naik, G. O., C. Forster, and P. W. Armstrong. “Measurement of angiotensin II: use of two injectors to minimize HPLC shadowing.” Peptides 15, no. 7 (1994): 1255–59. https://doi.org/10.1016/0196-9781(94)90150-3.
Naik GO, Forster C, Armstrong PW. Measurement of angiotensin II: use of two injectors to minimize HPLC shadowing. Peptides. 1994;15(7):1255–9.
Naik, G. O., et al. “Measurement of angiotensin II: use of two injectors to minimize HPLC shadowing.” Peptides, vol. 15, no. 7, 1994, pp. 1255–59. Pubmed, doi:10.1016/0196-9781(94)90150-3.
Naik GO, Forster C, Armstrong PW. Measurement of angiotensin II: use of two injectors to minimize HPLC shadowing. Peptides. 1994;15(7):1255–1259.
Published In
Peptides
DOI
ISSN
0196-9781
Publication Date
1994
Volume
15
Issue
7
Start / End Page
1255 / 1259
Location
United States
Related Subject Headings
- Reference Standards
- Myocardium
- Male
- Dogs
- Chromatography, High Pressure Liquid
- Animals
- Angiotensins
- Angiotensin II