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Rapid high-performance liquid chromatographic analysis of amiodarone and N-desethyl-amiodarone in serum.

Publication ,  Journal Article
Brien, JF; Jimmo, S; Armstrong, PW
Published in: Can J Physiol Pharmacol
March 1983

A rapid and reliable high-performance liquid chromatographic (HPLC) assay has been developed for the measurement of the antiarrhythmic drug, amiodarone (A), and its metabolite, N-desethyl-amiodarone (NDA), in serum. The procedure involves addition of absolute ethanol to serum, previously adjusted to pH 6, followed by centrifugation. The supernatant is analyzed by reverse-phase HPLC with ultraviolet-visible detection. There is no intereference from endogenous substances or other commonly used antiarrhythmic agents. The mean overall accuracy of the HPLC assay is 96.9 +/- 9.1 (SD)% (n = 37) for A over the concentration range 0.10-10.00 micrograms/mL, and 99.8 +/- 4.8 (SD)% (n = 32) for NDA over the concentration range 0.10-7.50 micrograms/mL. The within-day coefficient of variation is less than 7% for A and NDA. The lower limit of quantitative sensitivity is 0.10 micrograms/mL for A and NDA, and the lower limit of qualitative detection is 1.0 ng for both compounds.

Duke Scholars

Published In

Can J Physiol Pharmacol

DOI

ISSN

0008-4212

Publication Date

March 1983

Volume

61

Issue

3

Start / End Page

245 / 248

Location

Canada

Related Subject Headings

  • Physiology
  • Humans
  • Chromatography, High Pressure Liquid
  • Benzofurans
  • Amiodarone
  • 1116 Medical Physiology
  • 1115 Pharmacology and Pharmaceutical Sciences
 

Citation

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Brien, J. F., Jimmo, S., & Armstrong, P. W. (1983). Rapid high-performance liquid chromatographic analysis of amiodarone and N-desethyl-amiodarone in serum. Can J Physiol Pharmacol, 61(3), 245–248. https://doi.org/10.1139/y83-038
Brien, J. F., S. Jimmo, and P. W. Armstrong. “Rapid high-performance liquid chromatographic analysis of amiodarone and N-desethyl-amiodarone in serum.Can J Physiol Pharmacol 61, no. 3 (March 1983): 245–48. https://doi.org/10.1139/y83-038.
Brien JF, Jimmo S, Armstrong PW. Rapid high-performance liquid chromatographic analysis of amiodarone and N-desethyl-amiodarone in serum. Can J Physiol Pharmacol. 1983 Mar;61(3):245–8.
Brien, J. F., et al. “Rapid high-performance liquid chromatographic analysis of amiodarone and N-desethyl-amiodarone in serum.Can J Physiol Pharmacol, vol. 61, no. 3, Mar. 1983, pp. 245–48. Pubmed, doi:10.1139/y83-038.
Brien JF, Jimmo S, Armstrong PW. Rapid high-performance liquid chromatographic analysis of amiodarone and N-desethyl-amiodarone in serum. Can J Physiol Pharmacol. 1983 Mar;61(3):245–248.

Published In

Can J Physiol Pharmacol

DOI

ISSN

0008-4212

Publication Date

March 1983

Volume

61

Issue

3

Start / End Page

245 / 248

Location

Canada

Related Subject Headings

  • Physiology
  • Humans
  • Chromatography, High Pressure Liquid
  • Benzofurans
  • Amiodarone
  • 1116 Medical Physiology
  • 1115 Pharmacology and Pharmaceutical Sciences