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A c-Fos- and E1A-interacting component of the tissue factor basal promoter complex mediates synergistic activation of transcription by transforming growth factor-beta1.

Publication ,  Journal Article
Felts, SJ; Stang, MT; Getz, MJ
Published in: Oncogene
April 10, 1997

Stimulation of quiescent mouse fibroblasts with TGF-beta1 and certain other growth factors result in cooperative activation of tissue factor (TF) gene transcription, an event accompanied by the rapid entry of c-Fos into specific AP-1 DNA-binding complexes (Felts et al. (1995) Biochemistry 34, 12355-12362). Here, we demonstrate that the ability of TGF-beta1 to synergistically activate TF transcription in serum-stimulated fibroblasts is dependent upon both c-Fos and a promoter-specific factor with functional properties characteristic of transcriptional coactivators. Inhibition of TF promoter activity by an adenovirus E1A mutant deleted in an essential CREB binding protein (CBP) interaction domain suggests that this factor is distinct from the CBP/p300 family of transcriptional coactivators. Importantly, the ability of this factor to mediate molecular interactions with c-Fos required for transcriptional synergism is directly linked to TGF-beta1 signaling. These data suggest a model in which a component of the TF basal transcription complex functions to integrate multiple signaling pathways required for full transcriptional activation of TF in fibroblasts.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

April 10, 1997

Volume

14

Issue

14

Start / End Page

1679 / 1685

Location

England

Related Subject Headings

  • Transforming Growth Factor beta
  • Transcription Factors
  • Trans-Activators
  • Thromboplastin
  • Signal Transduction
  • Proto-Oncogene Proteins c-fos
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Nuclear Proteins
  • Mice
 

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

April 10, 1997

Volume

14

Issue

14

Start / End Page

1679 / 1685

Location

England

Related Subject Headings

  • Transforming Growth Factor beta
  • Transcription Factors
  • Trans-Activators
  • Thromboplastin
  • Signal Transduction
  • Proto-Oncogene Proteins c-fos
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Nuclear Proteins
  • Mice