CAR T Cells Targeting Podoplanin Reduce Orthotopic Glioblastomas in Mouse Brains.

Journal Article

Glioblastoma (GBM) is the most common and lethal primary malignant brain tumor in adults with a 5-year overall survival rate of less than 10%. Podoplanin (PDPN) is a type I transmembrane mucin-like glycoprotein, expressed in the lymphatic endothelium. Several solid tumors overexpress PDPN, including the mesenchymal type of GBM, which has been reported to present the worst prognosis among GBM subtypes. Chimeric antigen receptor (CAR)-transduced T cells can recognize predefined tumor surface antigens independent of MHC restriction, which is often downregulated in gliomas. We constructed a lentiviral vector expressing a third-generation CAR comprising a PDPN-specific antibody (NZ-1-based single-chain variable fragment) with CD28, 4-1BB, and CD3ζ intracellular domains. CAR-transduced peripheral blood monocytes were immunologically evaluated by calcein-mediated cytotoxic assay, ELISA, tumor size, and overall survival. The generated CAR T cells were specific and effective against PDPN-positive GBM cells in vitro. Systemic injection of the CAR T cells into an immunodeficient mouse model inhibited the growth of intracranial glioma xenografts in vivo. CAR T-cell therapy that targets PDPN would be a promising adoptive immunotherapy to treat mesenchymal GBM.

Full Text

Duke Authors

Cited Authors

  • Shiina, S; Ohno, M; Ohka, F; Kuramitsu, S; Yamamichi, A; Kato, A; Motomura, K; Tanahashi, K; Yamamoto, T; Watanabe, R; Ito, I; Senga, T; Hamaguchi, M; Wakabayashi, T; Kaneko, MK; Kato, Y; Chandramohan, V; Bigner, DD; Natsume, A

Published Date

  • March 2016

Published In

Volume / Issue

  • 4 / 3

Start / End Page

  • 259 - 268

PubMed ID

  • 26822025

Electronic International Standard Serial Number (EISSN)

  • 2326-6074

International Standard Serial Number (ISSN)

  • 2326-6066

Digital Object Identifier (DOI)

  • 10.1158/2326-6066.cir-15-0060

Language

  • eng