Photoinduced Electron Transfer Elicits a Change in the Static Dielectric Constant of a de Novo Designed Protein.

Journal Article

We provide a direct measure of the change in effective dielectric constant (ε(S)) within a protein matrix after a photoinduced electron transfer (ET) reaction. A linked donor-bridge-acceptor molecule, PZn-Ph-NDI, consisting of a (porphinato)Zn donor (PZn), a phenyl bridge (Ph), and a naphthalene diimide acceptor (NDI), is shown to be a "meter" to indicate protein dielectric environment. We calibrated PZn-Ph-NDI ET dynamics as a function of solvent dielectric, and computationally de novo designed a protein SCPZnI3 to bind PZn-Ph-NDI in its interior. Mapping the protein ET dynamics onto the calibrated ET catalogue shows that SCPZnI3 undergoes a switch in the effective dielectric constant following photoinduced ET, from ε(S) ≈ 8 to ε(S) ≈ 3.

Full Text

Duke Authors

Cited Authors

  • Polizzi, NF; Eibling, MJ; Perez-Aguilar, JM; Rawson, J; Lanci, CJ; Fry, HC; Beratan, DN; Saven, JG; Therien, MJ

Published Date

  • February 3, 2016

Published In

Volume / Issue

  • 138 / 7

Start / End Page

  • 2130 - 2133

PubMed ID

  • 26840013

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/jacs.5b13180

Language

  • eng