PCR-Based Analysis of Mitochondrial DNA Copy Number, Mitochondrial DNA Damage, and Nuclear DNA Damage.

Published

Journal Article

Because of the role that DNA damage and depletion play in human disease, it is important to develop and improve tools to assess these endpoints. This unit describes PCR-based methods to measure nuclear and mitochondrial DNA damage and copy number. Long amplicon quantitative polymerase chain reaction (LA-QPCR) is used to detect DNA damage by measuring the number of polymerase-inhibiting lesions present based on the amount of PCR amplification; real-time PCR (RT-PCR) is used to calculate genome content. In this unit, we provide step-by-step instructions to perform these assays in Homo sapiens, Mus musculus, Rattus norvegicus, Caenorhabditis elegans, Drosophila melanogaster, Danio rerio, Oryzias latipes, Fundulus grandis, and Fundulus heteroclitus, and discuss the advantages and disadvantages of these assays.

Full Text

Duke Authors

Cited Authors

  • Gonzalez-Hunt, CP; Rooney, JP; Ryde, IT; Anbalagan, C; Joglekar, R; Meyer, JN

Published Date

  • February 2016

Published In

Volume / Issue

  • 67 /

Start / End Page

  • 20.11.1 - 20.11.25

PubMed ID

  • 26828332

Pubmed Central ID

  • 26828332

Electronic International Standard Serial Number (EISSN)

  • 1934-9262

International Standard Serial Number (ISSN)

  • 1934-9254

Digital Object Identifier (DOI)

  • 10.1002/0471140856.tx2011s67

Language

  • eng