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Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.

Publication ,  Journal Article
Noviani, M; Jamiolkowski, RM; Grenet, JE; Lin, Q; Carlon, TA; Qi, L; Jantzen, AE; Milano, CA; Truskey, GA; Achneck, HE
Published in: ASAIO J
2016

The most promising alternatives to heart transplantation are left ventricular assist devices and artificial hearts; however, their use has been limited by thrombotic complications. To reduce these, sintered titanium (Ti) surfaces were developed, but thrombosis still occurs in approximately 7.5% of patients. We have invented a rapid-seeding technology to minimize the risk of thrombosis by rapid endothelialization of sintered Ti with human cord blood-derived endothelial cells (hCB-ECs). Human cord blood-derived endothelial cells were seeded within minutes onto sintered Ti and exposed to thrombosis-prone low fluid flow shear stresses. The hCB-ECs adhered and formed a confluent endothelial monolayer on sintered Ti. The exposure of sintered Ti to 4.4 dynes/cm for 20 hr immediately after rapid seeding resulted in approximately 70% cell adherence. The cell adherence was not significantly increased by additional ex vivo static culture of rapid-seeded sintered Ti before flow exposure. In addition, adherent hCB-ECs remained functional on sintered Ti, as indicated by flow-induced increase in nitric oxide secretion and reduction in platelet adhesion. After 15 day ex vivo static culture, the adherent hCB-ECs remained metabolically active, expressed endothelial cell functional marker thrombomodulin, and reduced platelet adhesion. In conclusion, our results demonstrate the feasibility of rapid-seeding sintered Ti with blood-derived hCB-ECs to generate a living antithrombotic surface.

Duke Scholars

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Published In

ASAIO J

DOI

EISSN

1538-943X

Publication Date

2016

Volume

62

Issue

4

Start / End Page

447 / 453

Location

United States

Related Subject Headings

  • Titanium
  • Thrombosis
  • Point-of-Care Systems
  • Platelet Adhesiveness
  • Humans
  • Heart-Assist Devices
  • Fetal Blood
  • Endothelial Cells
  • Cells, Cultured
  • Biomedical Engineering
 

Citation

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Noviani, M., Jamiolkowski, R. M., Grenet, J. E., Lin, Q., Carlon, T. A., Qi, L., … Achneck, H. E. (2016). Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating. ASAIO J, 62(4), 447–453. https://doi.org/10.1097/MAT.0000000000000351
Noviani, Maria, Ryan M. Jamiolkowski, Justin E. Grenet, Qiuyu Lin, Tim A. Carlon, Le Qi, Alexandra E. Jantzen, Carmelo A. Milano, George A. Truskey, and Hardean E. Achneck. “Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.ASAIO J 62, no. 4 (2016): 447–53. https://doi.org/10.1097/MAT.0000000000000351.
Noviani M, Jamiolkowski RM, Grenet JE, Lin Q, Carlon TA, Qi L, et al. Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating. ASAIO J. 2016;62(4):447–53.
Noviani, Maria, et al. “Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.ASAIO J, vol. 62, no. 4, 2016, pp. 447–53. Pubmed, doi:10.1097/MAT.0000000000000351.
Noviani M, Jamiolkowski RM, Grenet JE, Lin Q, Carlon TA, Qi L, Jantzen AE, Milano CA, Truskey GA, Achneck HE. Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating. ASAIO J. 2016;62(4):447–453.

Published In

ASAIO J

DOI

EISSN

1538-943X

Publication Date

2016

Volume

62

Issue

4

Start / End Page

447 / 453

Location

United States

Related Subject Headings

  • Titanium
  • Thrombosis
  • Point-of-Care Systems
  • Platelet Adhesiveness
  • Humans
  • Heart-Assist Devices
  • Fetal Blood
  • Endothelial Cells
  • Cells, Cultured
  • Biomedical Engineering