PCR based determination of mitochondrial DNA copy number in multiple species


Journal Article

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.

Full Text

Duke Authors

Cited Authors

  • Rooney, JP; Ryde, IT; Sanders, LH; Howlett, EH; Colton, MD; Germ, KE; Mayer, GD; Greenamyre, JT; Meyer, JN

Published Date

  • January 1, 2015

Published In

Volume / Issue

  • 1241 /

Start / End Page

  • 23 - 38

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-1875-1_3

Citation Source

  • Scopus