Dispersion-based stimulated Raman scattering spectroscopy, holography, and optical coherence tomography.

Journal Article

Stimulated Raman scattering (SRS) enables fast, high resolution imaging of chemical constituents important to biological structures and functional processes, both in a label-free manner and using exogenous biomarkers. While this technology has shown remarkable potential, it is currently limited to point scanning and can only probe a few Raman bands at a time (most often, only one). In this work we take a fundamentally different approach to detecting the small nonlinear signals based on dispersion effects that accompany the loss/gain processes in SRS. In this proof of concept, we demonstrate that the dispersive measurements are more robust to noise compared to amplitude-based measurements, which then permit spectral or spatial multiplexing (potentially both, simultaneously). Finally, we illustrate how this method may enable different strategies for biochemical imaging using phase microscopy and optical coherence tomography.

Full Text

Duke Authors

Cited Authors

  • Robles, FE; Fischer, MC; Warren, WS

Published Date

  • January 2016

Published In

Volume / Issue

  • 24 / 1

Start / End Page

  • 485 - 498

PubMed ID

  • 26832279

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

International Standard Serial Number (ISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/oe.24.000485

Language

  • eng