The location of olfactory receptors within olfactory epithelium is independent of odorant volatility and solubility.

Published online

Journal Article

BACKGROUND: Our objective was to study the pattern of olfactory receptor expression within the dorsal and ventral regions of the mouse olfactory epithelium. We hypothesized that olfactory receptors were distributed based on the chemical properties of their ligands: e.g. receptors for polar, hydrophilic and weakly volatile odorants would be present in the dorsal region of olfactory epithelium; while receptors for non-polar, more volatile odorants would be distributed to the ventral region. To test our hypothesis, we used micro-transplantation of cilia-enriched plasma membranes derived from dorsal or ventral regions of the olfactory epithelium into Xenopus oocytes for electrophysiological characterization against a panel of 100 odorants. FINDINGS: Odorants detected by ORs from the dorsal and ventral regions showed overlap in volatility and water solubility. We did not find evidence for a correlation between the solubility and volatility of odorants and the functional expression of olfactory receptors in the dorsal or ventral region of the olfactory epithelia. CONCLUSIONS: No simple clustering or relationship between chemical properties of odorants could be associated with the different regions of the olfactory epithelium. These results suggest that the location of ORs within the epithelium is not organized based on the physico-chemical properties of their ligands.

Full Text

Duke Authors

Cited Authors

  • Abaffy, T; Defazio, AR

Published Date

  • May 6, 2011

Published In

Volume / Issue

  • 4 /

Start / End Page

  • 137 -

PubMed ID

  • 21548958

Pubmed Central ID

  • 21548958

Electronic International Standard Serial Number (EISSN)

  • 1756-0500

Digital Object Identifier (DOI)

  • 10.1186/1756-0500-4-137

Language

  • eng

Conference Location

  • England