Differential volatile signatures from skin, naevi and melanoma: a novel approach to detect a pathological process.

Published online

Journal Article

BACKGROUND: Early detection of melanoma is of great importance to reduce mortality. Discovering new melanoma biomarkers would improve early detection and diagnosis. Here, we present a novel approach to detect volatile compounds from skin. METHODS AND FINDINGS: We used Head Space Solid Phase Micro-Extraction (HS-SPME) and gas chromatography/mass spectrometry (GC/MS) to identify volatile signatures from melanoma, naevi and skin samples. We hypothesized that the metabolic state of tissue alters the profile of volatile compounds. Volatiles released from fresh biopsy tissue of melanoma and benign naevus were compared based on their difference in frequency distribution and their expression level. We also analyzed volatile profiles from frozen tissue, including skin and melanoma. CONCLUSIONS: Three volatiles, 4-methyl decane, dodecane and undecane were preferentially expressed in both fresh and frozen melanoma, indicating that they are candidate biomarkers. Twelve candidate biomarkers evaluated by fuzzy logic analysis of frozen samples distinguished melanoma from skin with 89% sensitivity and 90% specificity. Our results demonstrate proof-of-principle that there is differential expression of volatiles in melanoma. Our volatile metabolomic approach will lead to a better understanding of melanoma and can enable development of new diagnostic and treatment strategies based on altered metabolism.

Full Text

Duke Authors

Cited Authors

  • Abaffy, T; Duncan, R; Riemer, DD; Tietje, O; Elgart, G; Milikowski, C; DeFazio, RA

Published Date

  • November 4, 2010

Published In

Volume / Issue

  • 5 / 11

Start / End Page

  • e13813 -

PubMed ID

  • 21079799

Pubmed Central ID

  • 21079799

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0013813

Language

  • eng

Conference Location

  • United States