A Fluorescence-Guided Laser Ablation System for Removal of Residual Cancer in a Mouse Model of Soft Tissue Sarcoma.

Published

Journal Article

The treatment of soft tissue sarcoma (STS) generally involves tumor excision with a wide margin. Although advances in fluorescence imaging make real-time detection of cancer possible, removal is limited by the precision of the human eye and hand. Here, we describe a novel pulsed Nd:YAG laser ablation system that, when used in conjunction with a previously described molecular imaging system, can identify and ablate cancer in vivo. Mice with primary STS were injected with the protease-activatable probe LUM015 to label tumors. Resected tissues from the mice were then imaged and treated with the laser using the paired fluorescence-imaging/ laser ablation device, generating ablation clefts with sub-millimeter precision and minimal underlying tissue damage. Laser ablation was guided by fluorescence to target tumor tissues, avoiding normal structures. The selective ablation of tumor implants in vivo improved recurrence-free survival after tumor resection in a cohort of 14 mice compared to 12 mice that received no ablative therapy. This prototype system has the potential to be modified so that it can be used during surgery to improve recurrence-free survival in patients with cancer.

Full Text

Duke Authors

Cited Authors

  • Lazarides, AL; Whitley, MJ; Strasfeld, DB; Cardona, DM; Ferrer, JM; Mueller, JL; Fu, HL; Bartholf DeWitt, S; Brigman, BE; Ramanujam, N; Kirsch, DG; Eward, WC

Published Date

  • January 2016

Published In

Volume / Issue

  • 6 / 2

Start / End Page

  • 155 - 166

PubMed ID

  • 26877775

Pubmed Central ID

  • 26877775

Electronic International Standard Serial Number (EISSN)

  • 1838-7640

International Standard Serial Number (ISSN)

  • 1838-7640

Digital Object Identifier (DOI)

  • 10.7150/thno.13536

Language

  • eng