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MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex.

Publication ,  Journal Article
Zhang, W; Kim, PJ; Chen, Z; Lokman, H; Qiu, L; Zhang, K; Rozen, SG; Tan, EK; Je, HS; Zeng, L
Published in: Elife
February 17, 2016

During the development, tight regulation of the expansion of neural progenitor cells (NPCs) and their differentiation into neurons is crucial for normal cortical formation and function. In this study, we demonstrate that microRNA (miR)-128 regulates the proliferation and differentiation of NPCs by repressing pericentriolar material 1 (PCM1). Specifically, overexpression of miR-128 reduced NPC proliferation but promoted NPC differentiation into neurons both in vivo and in vitro. In contrast, the reduction of endogenous miR-128 elicited the opposite effects. Overexpression of miR-128 suppressed the translation of PCM1, and knockdown of endogenous PCM1 phenocopied the observed effects of miR-128 overexpression. Furthermore, concomitant overexpression of PCM1 and miR-128 in NPCs rescued the phenotype associated with miR-128 overexpression, enhancing neurogenesis but inhibiting proliferation, both in vitro and in utero. Taken together, these results demonstrate a novel mechanism by which miR-128 regulates the proliferation and differentiation of NPCs in the developing neocortex.

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Published In

Elife

DOI

EISSN

2050-084X

Publication Date

February 17, 2016

Volume

5

Location

England

Related Subject Headings

  • Neural Stem Cells
  • MicroRNAs
  • Mice, Inbred C57BL
  • Gene Expression Regulation
  • Cerebral Cortex
  • Cell Proliferation
  • Cell Differentiation
  • Cell Cycle Proteins
  • Animals
  • 42 Health sciences
 

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Zhang, W., Kim, P. J., Chen, Z., Lokman, H., Qiu, L., Zhang, K., … Zeng, L. (2016). MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex. Elife, 5. https://doi.org/10.7554/eLife.11324
Zhang, Wei, Paul Jong Kim, Zhongcan Chen, Hidayat Lokman, Lifeng Qiu, Ke Zhang, Steven George Rozen, Eng King Tan, Hyunsoo Shawn Je, and Li Zeng. “MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex.Elife 5 (February 17, 2016). https://doi.org/10.7554/eLife.11324.
Zhang W, Kim PJ, Chen Z, Lokman H, Qiu L, Zhang K, et al. MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex. Elife. 2016 Feb 17;5.
Zhang, Wei, et al. “MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex.Elife, vol. 5, Feb. 2016. Pubmed, doi:10.7554/eLife.11324.
Zhang W, Kim PJ, Chen Z, Lokman H, Qiu L, Zhang K, Rozen SG, Tan EK, Je HS, Zeng L. MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex. Elife. 2016 Feb 17;5.

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

February 17, 2016

Volume

5

Location

England

Related Subject Headings

  • Neural Stem Cells
  • MicroRNAs
  • Mice, Inbred C57BL
  • Gene Expression Regulation
  • Cerebral Cortex
  • Cell Proliferation
  • Cell Differentiation
  • Cell Cycle Proteins
  • Animals
  • 42 Health sciences