3D TOCSY-HSQC NMR for Metabolic Flux Analysis Using Non-Uniform Sampling.

Published

Journal Article

(13)C-Metabolic Flux Analysis ((13)C-MFA) is rapidly being recognized as the authoritative method for determining fluxes through metabolic networks. Site-specific (13)C enrichment information obtained using NMR spectroscopy is a valuable input for (13)C-MFA experiments. Chemical shift overlaps in the 1D or 2D NMR experiments typically used for (13)C-MFA frequently hinder assignment and quantitation of site-specific (13)C enrichment. Here we propose the use of a 3D TOCSY-HSQC experiment for (13)C-MFA. We employ Non-Uniform Sampling (NUS) to reduce the acquisition time of the experiment to a few hours, making it practical for use in (13)C-MFA experiments. Our data show that the NUS experiment is linear and quantitative. Identification of metabolites in complex mixtures, such as a biomass hydrolysate, is simplified by virtue of the (13)C chemical shift obtained in the experiment. In addition, the experiment reports (13)C-labeling information that reveals the position specific labeling of subsets of isotopomers. The information provided by this technique will enable more accurate estimation of metabolic fluxes in large metabolic networks.

Full Text

Duke Authors

Cited Authors

  • Reardon, PN; Marean-Reardon, CL; Bukovec, MA; Coggins, BE; Isern, NG

Published Date

  • March 1, 2016

Published In

Volume / Issue

  • 88 / 5

Start / End Page

  • 2825 - 2831

PubMed ID

  • 26849182

Pubmed Central ID

  • 26849182

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

Digital Object Identifier (DOI)

  • 10.1021/acs.analchem.5b04535

Language

  • eng

Conference Location

  • United States