Analysis of the Rab GTPase Interactome in Dendritic Cells Reveals Anti-microbial Functions of the Rab32 Complex in Bacterial Containment.

Published

Journal Article

Dendritic cells (DCs) orchestrate complex membrane trafficking through an interconnected transportation network linked together by Rab GTPases. Through a tandem affinity purification strategy and mass spectrometry, we depicted an interactomic landscape of major members of the mammalian Rab GTPase family. When complemented with imaging tools, this proteomic analysis provided a global view of intracellular membrane organization. Driven by this analysis, we investigated dynamic changes to the Rab32 subnetwork in DCs induced by L. monocytogenes infection and uncovered an essential role of this subnetwork in controlling the intracellular proliferation of L. monocytogenes. Mechanistically, Rab32 formed a persistent complex with two interacting proteins, PHB and PHB2, to encompass bacteria both during early phagosome formation and after L. monocytogenes escaped the original containment vacuole. Collectively, we have provided a functional compartmentalization overview and an organizational framework of intracellular Rab-mediated vesicle trafficking that can serve as a resource for future investigations.

Full Text

Duke Authors

Cited Authors

  • Li, Y; Wang, Y; Zou, L; Tang, X; Yang, Y; Ma, L; Jia, Q; Ni, Q; Liu, S; Tang, L; Lin, R; Wong, E; Sun, W; Wang, L; Wei, Q; Ran, H; Zhang, L; Lian, H; Huang, W; Wu, Y; Li, Q-J; Wan, Y

Published Date

  • February 16, 2016

Published In

Volume / Issue

  • 44 / 2

Start / End Page

  • 422 - 437

PubMed ID

  • 26885862

Pubmed Central ID

  • 26885862

Electronic International Standard Serial Number (EISSN)

  • 1097-4180

Digital Object Identifier (DOI)

  • 10.1016/j.immuni.2016.01.027

Language

  • eng

Conference Location

  • United States