Transdifferentiation of human endothelial progenitors into smooth muscle cells.

Published

Journal Article

Access to smooth muscle cells (SMC) would create opportunities for tissue engineering, drug testing, and disease modeling. Herein we report the direct conversion of human endothelial progenitor cells (EPC) to induced smooth muscle cells (iSMC) by induced expression of MYOCD. The EPC undergo a cytoskeletal rearrangement resembling that of mesenchymal cells within 3 days post initiation of MYOCD expression. By day 7, the reprogrammed cells show upregulation of smooth muscle markers ACTA2, MYH11, and TAGLN by qRT-PCR and ACTA2 and MYH11 expression by immunofluorescence. By two weeks, they resemble umbilical artery SMC in microarray gene expression analysis. The iSMC, in contrast to EPC control, show calcium transients in response to phenylephrine stimulation and a contractility an order of magnitude higher than that of EPC as determined by traction force microscopy. Tissue-engineered blood vessels constructed using iSMC show functionality with respect to flow- and drug-mediated vasodilation and vasoconstriction.

Full Text

Duke Authors

Cited Authors

  • Ji, H; Atchison, L; Chen, Z; Chakraborty, S; Jung, Y; Truskey, GA; Christoforou, N; Leong, KW

Published Date

  • April 2016

Published In

Volume / Issue

  • 85 /

Start / End Page

  • 180 - 194

PubMed ID

  • 26874281

Pubmed Central ID

  • 26874281

Electronic International Standard Serial Number (EISSN)

  • 1878-5905

International Standard Serial Number (ISSN)

  • 0142-9612

Digital Object Identifier (DOI)

  • 10.1016/j.biomaterials.2016.01.066

Language

  • eng