Surface Functionalized Graphene Biosensor on Sapphire for Cancer Cell Detection.


Journal Article

Graphene has several unique physical, optical and electrical properties such as a two-dimensional (2D) planar structure, high optical transparency and high carrier mobility at room temperature. These make graphene interesting for electrical biosensing. Using a catalyst-free chemical vapor deposition (CVD) method, graphene film is grown on a sapphire substrate. There is a single or a few sheets as confirmed by Raman spectroscopy and atomic force microscopy (AFM). Electrical graphene biosensors are fabricated to detect large-sized biological analytes such as cancer cells. Human colorectal carcinoma cells are sensed by the resistance change of an active bio-functionalized graphene device as the cells are captured by the immobilized antibody surface. The functionalized sensors show an increase in resistance as large as ~20% of the baseline with a small number of adhered cells. This study suggests that the bio-functionalized electrical graphene sensors on sapphire, which is a highly transparent material, can potentially detect circulating tumor cells (CTCs) and monitor cellular electrical behavior while being compatible with fluorescence-based optical-detection bioassays.

Full Text

Duke Authors

Cited Authors

  • Joe, DJ; Hwang, J; Johnson, C; Cha, H-Y; Lee, J-W; Shen, X; Spencer, MG; Tiwari, S; Kim, M

Published Date

  • January 2016

Published In

Volume / Issue

  • 16 / 1

Start / End Page

  • 144 - 151

PubMed ID

  • 27398439

Pubmed Central ID

  • 27398439

Electronic International Standard Serial Number (EISSN)

  • 1533-4899

International Standard Serial Number (ISSN)

  • 1533-4880

Digital Object Identifier (DOI)

  • 10.1166/jnn.2016.12042


  • eng