Diagnostic methods to determine microbiology of postpartum endometritis in South Asia: laboratory methods protocol used in the Postpartum Sepsis Study: a prospective cohort study.

Journal Article (Journal Article;Multicenter Study)

BACKGROUND: The South Asian region has the second highest risk of maternal death in the world. To prevent maternal deaths due to sepsis and to decrease the maternal mortality ratio as per the World Health Organization Millenium Development Goals, a better understanding of the etiology of endometritis and related sepsis is required. We describe microbiological laboratory methods used in the maternal Postpartum Sepsis Study, which was conducted in Bangladesh and Pakistan, two populous countries in South Asia. METHODS/DESIGN: Postpartum maternal fever in the community was evaluated by a physician and blood and urine were collected for routine analysis and culture. If endometritis was suspected, an endometrial brush sample was collected in the hospital for aerobic and anaerobic culture and molecular detection of bacterial etiologic agents (previously identified and/or plausible). DISCUSSION: The results emanating from this study will provide microbiologic evidence of the etiology and susceptibility pattern of agents recovered from patients with postpartum fever in South Asia, data critical for the development of evidence-based algorithms for management of postpartum fever in the region.

Full Text

Duke Authors

Cited Authors

  • Shakoor, S; Reller, ME; LeFevre, A; Hotwani, A; Qureshi, SM; Yousuf, F; Islam, MS; Connor, N; Rafiqullah, I; Mir, F; Arif, S; Soofi, S; Bartlett, LA; Saha, S; ANISA-Postpartum Sepsis Study Group,

Published Date

  • February 25, 2016

Published In

Volume / Issue

  • 13 /

Start / End Page

  • 15 -

PubMed ID

  • 26916013

Pubmed Central ID

  • PMC4766642

Electronic International Standard Serial Number (EISSN)

  • 1742-4755

Digital Object Identifier (DOI)

  • 10.1186/s12978-016-0121-4


  • eng

Conference Location

  • England