Gradated assembly of multiple proteins into supramolecular nanomaterials.

Journal Article

Biomaterials exhibiting precise ratios of different bioactive protein components are critical for applications ranging from vaccines to regenerative medicine, but their design is often hindered by limited choices and cross-reactivity of protein conjugation chemistries. Here, we describe a strategy for inducing multiple different expressed proteins of choice to assemble into nanofibres and gels with exceptional compositional control. The strategy employs 'βTail' tags, which allow for good protein expression in bacteriological cultures, yet can be induced to co-assemble into nanomaterials when mixed with additional β-sheet fibrillizing peptides. Multiple different βTail fusion proteins could be inserted into peptide nanofibres alone or in combination at predictable, smoothly gradated concentrations, providing a simple yet versatile route to install precise combinations of proteins into nanomaterials. The technology is illustrated by achieving precisely targeted hues using mixtures of fluorescent proteins, by creating nanofibres bearing enzymatic activity, and by adjusting antigenic dominance in vaccines.

Full Text

Duke Authors

Cited Authors

  • Hudalla, GA; Sun, T; Gasiorowski, JZ; Han, H; Tian, YF; Chong, AS; Collier, JH

Published Date

  • August 2014

Published In

Volume / Issue

  • 13 / 8

Start / End Page

  • 829 - 836

PubMed ID

  • 24930032

International Standard Serial Number (ISSN)

  • 1476-1122

Digital Object Identifier (DOI)

  • 10.1038/nmat3998

Language

  • eng