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Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation.

Publication ,  Journal Article
Jung, JP; Jones, JL; Cronier, SA; Collier, JH
Published in: Biomaterials
May 2008

Hydrogels produced from self-assembling peptides and peptide derivatives are being investigated as synthetic extracellular matrices for defined cell culture substrates and scaffolds for regenerative medicine. In many cases, however, they are less stiff than the tissues and extracellular matrices they are intended to mimic, and they are prone to cohesive failure. We employed native chemical ligation to produce peptide bonds between the termini of fibrillized beta-sheet peptides to increase gel stiffness in a chemically specific manner while maintaining the morphology of the self-assembled fibrils. Polymerization, fibril structure, and mechanical properties were measured by SDS-PAGE, mass spectrometry, TEM, circular dichroism, and oscillating rheometry; and cellular responses to matrix stiffening were investigated in cultures of human umbilical vein endothelial cells (HUVECs). Ligation led to a fivefold increase in storage modulus and a significant enhancement of HUVEC proliferation and expression of CD31 on the surface of the gels. The approach was also orthogonal to the inclusion of unprotected RGD-functionalized self-assembling peptides, which further increased proliferation. This strategy broadens the utility of self-assembled peptide materials for applications that require enhancement or modulation of matrix mechanical properties by providing a chemoselective means for doing so without significantly disrupting the gels' fibrillar structure.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

May 2008

Volume

29

Issue

13

Start / End Page

2143 / 2151

Related Subject Headings

  • Viscosity
  • Polymers
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Peptides
  • Osmolar Concentration
  • Microscopy, Electron, Transmission
  • Hydrogels
  • Humans
  • Endothelial Cells
  • Elasticity
 

Citation

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Jung, J. P., Jones, J. L., Cronier, S. A., & Collier, J. H. (2008). Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation. Biomaterials, 29(13), 2143–2151. https://doi.org/10.1016/j.biomaterials.2008.01.008
Jung, Jangwook P., Julia L. Jones, Samantha A. Cronier, and Joel H. Collier. “Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation.Biomaterials 29, no. 13 (May 2008): 2143–51. https://doi.org/10.1016/j.biomaterials.2008.01.008.
Jung JP, Jones JL, Cronier SA, Collier JH. Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation. Biomaterials. 2008 May;29(13):2143–51.
Jung, Jangwook P., et al. “Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation.Biomaterials, vol. 29, no. 13, May 2008, pp. 2143–51. Epmc, doi:10.1016/j.biomaterials.2008.01.008.
Jung JP, Jones JL, Cronier SA, Collier JH. Modulating the mechanical properties of self-assembled peptide hydrogels via native chemical ligation. Biomaterials. 2008 May;29(13):2143–2151.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

May 2008

Volume

29

Issue

13

Start / End Page

2143 / 2151

Related Subject Headings

  • Viscosity
  • Polymers
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Peptides
  • Osmolar Concentration
  • Microscopy, Electron, Transmission
  • Hydrogels
  • Humans
  • Endothelial Cells
  • Elasticity