Controllably degradable β-sheet nanofibers and gels from self-assembling depsipeptides.

Published

Journal Article

Self-assembled peptide materials have received considerable interest for a range of applications, including 3D cell culture, tissue engineering, and the delivery of cells and drugs. One challenge in applying such materials within these areas has been the extreme stability of β-sheet fibrillized peptides, which are resistant to proteolysis, degradation, and turnover in biological environments. In this study, we designed self-assembling depsipeptides containing ester bonds within the peptide backbone. Beta-sheet fibrillized nanofibers were formed in physiologic conditions, and two of these nanofiber-forming depsipeptides produced hydrogels that degraded controllably over the course of days-to-weeks via ester hydrolysis. With HPLC, TEM, and oscillating rheometry, we show that the rate of hydrolysis can be controlled in a straightforward manner by specifying the amino acid residues surrounding the ester bond. In 3D cell cultures, depsipeptide gels softened over the course of several days and permitted considerably more proliferation and spreading of C3H10T1/2 pluripotent stem cells than non-degradable analogs. This approach now provides a reliable and reproducible means to soften or clear β-sheet fibrillized peptide materials from biological environments.

Full Text

Duke Authors

Cited Authors

  • Tian, YF; Hudalla, GA; Han, H; Collier, JH

Published Date

  • October 2013

Published In

Volume / Issue

  • 1 / 10

PubMed ID

  • 24224082

Pubmed Central ID

  • 24224082

Electronic International Standard Serial Number (EISSN)

  • 2047-4849

International Standard Serial Number (ISSN)

  • 2047-4830

Digital Object Identifier (DOI)

  • 10.1039/c3bm60161g

Language

  • eng