Manufacture and characterization of poly(alpha-hydroxy ester) thin films as temporary substrates for retinal pigment epithelium cells.


Journal Article

For many disorders of the retinal pigment epithelium (RPE) for which there are no effective treatments, transplantation of RPE cells may provide a viable means of restoring function. Using a solvent casting technique, we have manufactured thin films of poly(L-lactic acid) and poly(DL-lactic-co-glycolic acid) 75:25 and 50:50. Non-porous, flexible films with controlled thickness as thin as 12 +/- 3 microns and reproducible surface morphologies and flexural properties were produced. Fetal human RPE cells were found to attach to these substrates when cultured in vitro. The films made using this technique may provide a means of transplanting allogeneic RPE cells as a therapy for a number of ocular diseases related to RPE dysfunction.

Full Text

Cited Authors

  • Thomson, RC; Giordano, GG; Collier, JH; Ishaug, SL; Mikos, AG; Lahiri-Munir, D; Garcia, CA

Published Date

  • February 1996

Published In

Volume / Issue

  • 17 / 3

Start / End Page

  • 321 - 327

PubMed ID

  • 8745329

Pubmed Central ID

  • 8745329

Electronic International Standard Serial Number (EISSN)

  • 1878-5905

International Standard Serial Number (ISSN)

  • 0142-9612

Digital Object Identifier (DOI)

  • 10.1016/0142-9612(96)85570-0


  • eng