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Co-assembling peptides as defined matrices for endothelial cells.

Publication ,  Journal Article
Jung, JP; Nagaraj, AK; Fox, EK; Rudra, JS; Devgun, JM; Collier, JH
Published in: Biomaterials
April 2009

Self-assembling peptides and peptide derivatives bearing cell-binding ligands are increasingly being investigated as defined cell culture matrices and as scaffolds for regenerative medicine. In order to systematically refine such scaffolds to elicit specific desired cell behaviors, ligand display should ideally be achieved without inadvertently altering other physicochemical properties such as viscoelasticity. Moreover, for in vivo applications, self-assembled biomaterials must exhibit low immunogenicity. In the present study, multi-peptide co-assembling hydrogels based on the beta-sheet fibrillizing peptide Q11 (QQKFQFQFEQQ) were designed such that they presented RGDS or IKVAV ligands on their fibril surfaces. In co-assemblies of the ligand-bearing peptides with Q11, ligand incorporation levels capable of influencing the attachment, spreading, morphology, and growth of human umbilical vein endothelial cells (HUVECs) did not significantly alter the materials' fibrillization, beta-turn secondary structure, or stiffness. RGDS-Q11 specifically increased HUVEC attachment, spreading, and growth when co-assembled into Q11 gels, whereas IKVAV-Q11 exerted a more subtle influence on attachment and morphology. Additionally, Q11 and RGDS-Q11 were minimally immunogenic in mice, making Q11-based biomaterials attractive candidates for further investigation as defined, modular extracellular matrices for applications in vitro and in vivo.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

April 2009

Volume

30

Issue

12

Start / End Page

2400 / 2410

Related Subject Headings

  • Viscosity
  • Peptides
  • Microscopy, Electron, Transmission
  • Mice, Inbred C57BL
  • Mice
  • Ligands
  • Humans
  • Gelatin
  • Female
  • Endothelial Cells
 

Citation

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Jung, J. P., Nagaraj, A. K., Fox, E. K., Rudra, J. S., Devgun, J. M., & Collier, J. H. (2009). Co-assembling peptides as defined matrices for endothelial cells. Biomaterials, 30(12), 2400–2410. https://doi.org/10.1016/j.biomaterials.2009.01.033
Jung, Jangwook P., Arun K. Nagaraj, Emily K. Fox, Jai S. Rudra, Jason M. Devgun, and Joel H. Collier. “Co-assembling peptides as defined matrices for endothelial cells.Biomaterials 30, no. 12 (April 2009): 2400–2410. https://doi.org/10.1016/j.biomaterials.2009.01.033.
Jung JP, Nagaraj AK, Fox EK, Rudra JS, Devgun JM, Collier JH. Co-assembling peptides as defined matrices for endothelial cells. Biomaterials. 2009 Apr;30(12):2400–10.
Jung, Jangwook P., et al. “Co-assembling peptides as defined matrices for endothelial cells.Biomaterials, vol. 30, no. 12, Apr. 2009, pp. 2400–10. Epmc, doi:10.1016/j.biomaterials.2009.01.033.
Jung JP, Nagaraj AK, Fox EK, Rudra JS, Devgun JM, Collier JH. Co-assembling peptides as defined matrices for endothelial cells. Biomaterials. 2009 Apr;30(12):2400–2410.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

April 2009

Volume

30

Issue

12

Start / End Page

2400 / 2410

Related Subject Headings

  • Viscosity
  • Peptides
  • Microscopy, Electron, Transmission
  • Mice, Inbred C57BL
  • Mice
  • Ligands
  • Humans
  • Gelatin
  • Female
  • Endothelial Cells