Short-term traffic related exposures and biomarkers of nitro-PAH exposure and oxidative DNA damage.

Journal Article

Exposure to vehicle exhaust has been associated with cardiac and respiratory disease, lung cancer, and greater overall mortality. We investigated whether amino- polycyclic aromatic hydrocarbon (amino-PAH) metabolites of nitro-PAHs could be used as biomarkers of these exposures. Pre- and post-shift urine samples were collected at the beginning and end of a work week from 82 male U.S trucking industry workers. We used repeated-measures analysis to examine associations of total 1- and 2-aminonaphthalene (1 & 2-AN) and 1-aminopyrene (1-AP) urinary concentrations with microenvironment exposures to particulate matter (PM2.5 ), elemental and organic carbon, and between 1&2-AN and 1-AP with urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG). There was an association between work week mean PM2.5 levels and post-shift 1 & 2-AN, [141.8 pg/ml increase (95% CI:53.3, 230.2) for each IQR increase (5.54 µg/m3 ) in PM2.5 ,] but no associations with other exposure measures. There was a statistically significant increase in 8-OHdG concentrations with 1 & 2-AN (2.38 µg/mg creatinine (95%CI: 0.19, 4.58) per 242.85 pg/mg creatinine increase in 1 & 2-AN), and suggestive associations with all other exposure measures. Our findings suggest associations between urinary amino-PAHs with vehicle exhaust related PM2.5 as well as with a biomarker of oxidative DNA damage.

Full Text

Duke Authors

Cited Authors

  • Neophytou, AM; Hart, JE; Chang, Y; Zhang, JJ; Smith, TJ; Garshick, E; Laden, F

Published Date

  • September 2014

Published In

Volume / Issue

  • 2 / 3

Start / End Page

  • 377 - 390

PubMed ID

  • 25254201

Pubmed Central ID

  • 25254201

Electronic International Standard Serial Number (EISSN)

  • 2305-6304

International Standard Serial Number (ISSN)

  • 2305-6304

Digital Object Identifier (DOI)

  • 10.3390/toxics2030377


  • eng