Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction.

Journal Article (Journal Article)

Salmonella enterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed. We validated a multiplex polymerase chain reaction (PCR) assay with the White-Kauffmann-Le Minor (WKLM) scheme of serotyping using 110 Salmonella isolates from blood cultures of febrile children in Ghana and applied the method in other Typhoid Fever Surveillance in Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) Salmonella enterica serovar Typhimurium, 14 (13%) Salmonella enterica serovar Dublin, and 13 (12%) Salmonella enterica serovar Enteritidis were identified by both multiplex PCR and the WKLM scheme separately. Using the validated multiplex PCR assay, we identified 42 (66%) S. Typhi, 14 (22%) S. Typhimurium, 2 (3%) S. Dublin, 2 (3%) S. Enteritidis, and 4 (6%) other Salmonella species from the febrile patients in Burkina Faso, Guinea-Bissau, Madagascar, Senegal, and Tanzania. Application of this multiplex PCR assay in sub-Saharan Africa could advance the knowledge of serotype distribution of Salmonella.

Full Text

Duke Authors

Cited Authors

  • Al-Emran, HM; Krumkamp, R; Dekker, DM; Eibach, D; Aaby, P; Adu-Sarkodie, Y; Ali, M; Rubach, MP; Bjerregaard-Andersen, M; Crump, JA; Cruz Espinoza, LM; Løfberg, SV; Gassama Sow, A; Hertz, JT; Im, J; Jaeger, A; Kabore, LP; Konings, F; Meyer, CG; Niang, A; Pak, GD; Panzner, U; Park, SE; Rabezanahary, H; Rakotozandrindrainy, R; Raminosoa, TM; Razafindrabe, TJL; Sampo, E; Schütt-Gerowitt, H; Sarpong, N; Soura, AB; Tall, A; von Kalckreuth, V; Wierzba, TF; May, J; Marks, F

Published Date

  • March 15, 2016

Published In

Volume / Issue

  • 62 Suppl 1 /

Start / End Page

  • S80 - S82

PubMed ID

  • 26933026

Electronic International Standard Serial Number (EISSN)

  • 1537-6591

Digital Object Identifier (DOI)

  • 10.1093/cid/civ782


  • eng

Conference Location

  • United States