A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes.

Journal Article (Journal Article)

Cellular functions of the Golgi are determined by the unique distribution of its resident proteins. Currently, electron microscopy is required for the localization of a Golgi protein at the sub-Golgi level. We developed a quantitative sub-Golgi localization method based on centers of fluorescence masses of nocodazole-induced Golgi ministacks under conventional optical microscopy. Our method is rapid, convenient, and quantitative, and it yields a practical localization resolution of ∼ 30 nm. The method was validated by the previous electron microscopy data. We quantitatively studied the intra-Golgi trafficking of synchronized secretory membrane cargoes and directly demonstrated the cisternal progression of cargoes from the cis- to the trans-Golgi. Our data suggest that the constitutive efflux of secretory cargoes could be restricted at the Golgi stack, and the entry of the trans-Golgi network in secretory pathway could be signal dependent.

Full Text

Duke Authors

Cited Authors

  • Tie, HC; Mahajan, D; Chen, B; Cheng, L; VanDongen, AMJ; Lu, L

Published Date

  • March 1, 2016

Published In

Volume / Issue

  • 27 / 5

Start / End Page

  • 848 - 861

PubMed ID

  • 26764092

Pubmed Central ID

  • PMC4803310

Electronic International Standard Serial Number (EISSN)

  • 1939-4586

Digital Object Identifier (DOI)

  • 10.1091/mbc.E15-09-0664


  • eng

Conference Location

  • United States