Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal 15N2-diazirine molecular tags

Journal Article

Conventional magnetic resonance (MR) faces serious sensitivity limitations which can be overcome by hyperpolarization methods, but the most common method (dynamic nuclear polarization) is complex and expensive, and applications are limited by short spin lifetimes (typically seconds) of biologically relevant molecules. We use a recently developed method, SABRE-SHEATH, to directly hyperpolarize 15N2 magnetization and long-lived 15N2 singlet spin order, with signal decay time constants of 5.8 and 23 minutes, respectively. We find >10,000-fold enhancements generating detectable nuclear MR signals that last for over an hour. 15N2-diazirines represent a class of particularly promising and versatile molecular tags, and can be incorporated into a wide range of biomolecules without significantly altering molecular function.

Full Text

Duke Authors

Cited Authors

  • Theis, T; Ortiz, GX; Logan, AWJ; Claytor, KE; Feng, Y; Huhn, WP; Blum, V; Malcolmson, SJ; Chekmenev, EY; Wang, Q; Warren, WS

Published Date

  • March 25, 2016

Published In

  • Science Advances

Volume / Issue

  • 2 / 3

PubMed ID

  • 27051867

Pubmed Central ID

  • PMC4820385

Digital Object Identifier (DOI)

  • 10.1126/sciadv.1501438