How the kinetochore couples microtubule force and centromere stretch to move chromosomes.

Published

Journal Article

The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-end polymerization and depolymerization. Spc24/Spc25 at one end of the Ndc80 complex binds the kinetochore. The N-terminal tail and CH domains of Ndc80 bind microtubules, and an internal domain binds microtubule-associated proteins (MAPs) such as the Dam1 complex. To determine how the microtubule- and MAP-binding domains of Ndc80 contribute to force production at the kinetochore in budding yeast, we have inserted a FRET tension sensor into the Ndc80 protein about halfway between its microtubule-binding and internal loop domains. The data support a mechanical model of force generation at metaphase where the position of the kinetochore relative to the microtubule plus end reflects the relative strengths of microtubule depolymerization, centromere stretch and microtubule-binding interactions with the Ndc80 and Dam1 complexes.

Full Text

Duke Authors

Cited Authors

  • Suzuki, A; Badger, BL; Haase, J; Ohashi, T; Erickson, HP; Salmon, ED; Bloom, K

Published Date

  • April 2016

Published In

Volume / Issue

  • 18 / 4

Start / End Page

  • 382 - 392

PubMed ID

  • 26974660

Pubmed Central ID

  • 26974660

Electronic International Standard Serial Number (EISSN)

  • 1476-4679

International Standard Serial Number (ISSN)

  • 1465-7392

Digital Object Identifier (DOI)

  • 10.1038/ncb3323

Language

  • eng