How the kinetochore couples microtubule force and centromere stretch to move chromosomes.
Journal Article (Journal Article)
The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-end polymerization and depolymerization. Spc24/Spc25 at one end of the Ndc80 complex binds the kinetochore. The N-terminal tail and CH domains of Ndc80 bind microtubules, and an internal domain binds microtubule-associated proteins (MAPs) such as the Dam1 complex. To determine how the microtubule- and MAP-binding domains of Ndc80 contribute to force production at the kinetochore in budding yeast, we have inserted a FRET tension sensor into the Ndc80 protein about halfway between its microtubule-binding and internal loop domains. The data support a mechanical model of force generation at metaphase where the position of the kinetochore relative to the microtubule plus end reflects the relative strengths of microtubule depolymerization, centromere stretch and microtubule-binding interactions with the Ndc80 and Dam1 complexes.
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Duke Authors
Cited Authors
- Suzuki, A; Badger, BL; Haase, J; Ohashi, T; Erickson, HP; Salmon, ED; Bloom, K
Published Date
- April 2016
Published In
Volume / Issue
- 18 / 4
Start / End Page
- 382 - 392
PubMed ID
- 26974660
Pubmed Central ID
- PMC4814359
Electronic International Standard Serial Number (EISSN)
- 1476-4679
Digital Object Identifier (DOI)
- 10.1038/ncb3323
Language
- eng
Conference Location
- England