Skip to main content
Journal cover image

Endothelial superoxide production is altered in sheep programmed by early gestation dexamethasone exposure.

Publication ,  Journal Article
Roghair, RD; Miller, FJ; Scholz, TD; Lamb, FS; Segar, JL
Published in: Neonatology
2008

BACKGROUND: Animal models have demonstrated that maternal undernutrition or early gestation glucocorticoid exposure induces endothelial dysfunction in the offspring. OBJECTIVES: We sought to determine whether early gestation dexamethasone (DEX) exposure is further associated with increased vascular superoxide anion production. METHODS: DEX (0.28 mg/kg/day i.v. for 48 h) was administered to pregnant ewes at 27-28 days' gestation (term 145 days). Tissues were harvested from DEX-exposed and control lambs at 125 days' gestation (n = 6 for each group) and 4 months following delivery (n = 9 and 12, respectively). RESULTS: By lucigenin-enhanced chemiluminescence, coronary and mesenteric arteries from DEX-exposed fetuses exhibited diminished basal superoxide production (both p < 0.01). Similarly, DEX-exposed carotid arteries from 4-month-old lambs had decreased superoxide production (p < 0.01) that localized to the endothelium by endothelial cell culture and dihydroethidium fluorescence. In contrast, DEX-exposed coronary arteries from the 4-month-old sheep had increased superoxide production (p < 0.05). Although early gestation DEX exposure did not alter lipid peroxidation, DEX exposure was associated with significantly increased renal and cerebral cortex aconitase activity (consistent with decreased protein oxidation). These changes occurred in the absence of alterations in renal cortex superoxide dismutase activity. CONCLUSIONS: We conclude that in a DEX-exposure model of fetal programming, endothelial superoxide production and protein oxidation are decreased in the mesenteric and carotid circulation. This contrasts with the postnatal coronary artery-specific increase in superoxide production.

Duke Scholars

Published In

Neonatology

DOI

EISSN

1661-7819

Publication Date

2008

Volume

93

Issue

1

Start / End Page

19 / 27

Location

Switzerland

Related Subject Headings

  • Vasodilation
  • Superoxides
  • Superoxide Dismutase
  • Sheep
  • Reactive Oxygen Species
  • Prenatal Exposure Delayed Effects
  • Pregnancy
  • Pediatrics
  • Oxidation-Reduction
  • Mesenteric Arteries
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Roghair, R. D., Miller, F. J., Scholz, T. D., Lamb, F. S., & Segar, J. L. (2008). Endothelial superoxide production is altered in sheep programmed by early gestation dexamethasone exposure. Neonatology, 93(1), 19–27. https://doi.org/10.1159/000105521
Roghair, Robert D., Francis J. Miller, Thomas D. Scholz, Fred S. Lamb, and Jeffrey L. Segar. “Endothelial superoxide production is altered in sheep programmed by early gestation dexamethasone exposure.Neonatology 93, no. 1 (2008): 19–27. https://doi.org/10.1159/000105521.
Roghair RD, Miller FJ, Scholz TD, Lamb FS, Segar JL. Endothelial superoxide production is altered in sheep programmed by early gestation dexamethasone exposure. Neonatology. 2008;93(1):19–27.
Roghair, Robert D., et al. “Endothelial superoxide production is altered in sheep programmed by early gestation dexamethasone exposure.Neonatology, vol. 93, no. 1, 2008, pp. 19–27. Pubmed, doi:10.1159/000105521.
Roghair RD, Miller FJ, Scholz TD, Lamb FS, Segar JL. Endothelial superoxide production is altered in sheep programmed by early gestation dexamethasone exposure. Neonatology. 2008;93(1):19–27.
Journal cover image

Published In

Neonatology

DOI

EISSN

1661-7819

Publication Date

2008

Volume

93

Issue

1

Start / End Page

19 / 27

Location

Switzerland

Related Subject Headings

  • Vasodilation
  • Superoxides
  • Superoxide Dismutase
  • Sheep
  • Reactive Oxygen Species
  • Prenatal Exposure Delayed Effects
  • Pregnancy
  • Pediatrics
  • Oxidation-Reduction
  • Mesenteric Arteries