Guiding intracortical brain tumour cells to an extracortical cytotoxic hydrogel using aligned polymeric nanofibres.

Published

Journal Article

Glioblastoma multiforme is an aggressive, invasive brain tumour with a poor survival rate. Available treatments are ineffective and some tumours remain inoperable because of their size or location. The tumours are known to invade and migrate along white matter tracts and blood vessels. Here, we exploit this characteristic of glioblastoma multiforme by engineering aligned polycaprolactone (PCL)-based nanofibres for tumour cells to invade and, hence, guide cells away from the primary tumour site to an extracortical location. This extracortial sink is a cyclopamine drug-conjugated, collagen-based hydrogel. When aligned PCL-nanofibre films in a PCL/polyurethane carrier conduit were inserted in the vicinity of an intracortical human U87MG glioblastoma xenograft, a significant number of human glioblastoma cells migrated along the aligned nanofibre films and underwent apoptosis in the extracortical hydrogel. Tumour volume in the brain was significantly lower following insertion of aligned nanofibre implants compared with the application of smooth fibres or no implants.

Full Text

Duke Authors

Cited Authors

  • Jain, A; Betancur, M; Patel, GD; Valmikinathan, CM; Mukhatyar, VJ; Vakharia, A; Pai, SB; Brahma, B; MacDonald, TJ; Bellamkonda, RV

Published Date

  • March 2014

Published In

Volume / Issue

  • 13 / 3

Start / End Page

  • 308 - 316

PubMed ID

  • 24531400

Pubmed Central ID

  • 24531400

International Standard Serial Number (ISSN)

  • 1476-1122

Digital Object Identifier (DOI)

  • 10.1038/nmat3878

Language

  • eng