Skip to main content
Journal cover image

Rational identification of a novel peptide for targeting nanocarriers to 9L glioma.

Publication ,  Journal Article
Agarwal, A; Jaye, DL; Giegerman, CM; Bellamkonda, RV
Published in: Journal of biomedical materials research. Part A
December 2008

Traditional therapies for high grade gliomas are limited in part by collateral damage to normal tissues. Selective delivery of therapies to tumors is, therefore, needed. Here, we report that liposomal nanocarriers coated with a novel oligopeptide enhance uptake by 9L gliosarcoma. A targeting nine amino acid peptide sequence (RSI) was identified by differential panning of random peptide phage display libraries on 9L cells and rat blood cells and plasma. Peptides were coupled to the surface of liposomal nanocarriers which were subsequently loaded with doxorubicin. The ability of RSI coated liposomes to facilitate drug uptake and cytotoxicity was compared with conventional liposomal nanocarriers and controls. In addition, plasma clearance profiles of the RSI peptide coupled liposomal nanocarriers were evaluated in adult immuno-competent rats. RSI peptide-coupled liposomal nanocarriers enhanced drug uptake by 9L cells by 500% compared with conventional liposomal nanocarriers, and significantly increased cytotoxicity. The plasma half-lives confirmed that the presence of the RSI peptide on the liposomal nanocarriers did not compromise circulation time in the blood in comparison with Stealth liposomal nanocarriers. These data suggest that phage-identified oligopeptides could lead to the development of new tumor selective nanocarriers.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Journal of biomedical materials research. Part A

DOI

EISSN

1552-4965

ISSN

1549-3296

Publication Date

December 2008

Volume

87

Issue

3

Start / End Page

728 / 738

Related Subject Headings

  • Tumor Cells, Cultured
  • Rats, Inbred F344
  • Rats
  • Peptides
  • Nanostructures
  • Male
  • Liposomes
  • Gliosarcoma
  • Drug Carriers
  • Doxorubicin
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Agarwal, A., Jaye, D. L., Giegerman, C. M., & Bellamkonda, R. V. (2008). Rational identification of a novel peptide for targeting nanocarriers to 9L glioma. Journal of Biomedical Materials Research. Part A, 87(3), 728–738. https://doi.org/10.1002/jbm.a.31762
Agarwal, Abhiruchi, David L. Jaye, Cissy M. Giegerman, and Ravi V. Bellamkonda. “Rational identification of a novel peptide for targeting nanocarriers to 9L glioma.Journal of Biomedical Materials Research. Part A 87, no. 3 (December 2008): 728–38. https://doi.org/10.1002/jbm.a.31762.
Agarwal A, Jaye DL, Giegerman CM, Bellamkonda RV. Rational identification of a novel peptide for targeting nanocarriers to 9L glioma. Journal of biomedical materials research Part A. 2008 Dec;87(3):728–38.
Agarwal, Abhiruchi, et al. “Rational identification of a novel peptide for targeting nanocarriers to 9L glioma.Journal of Biomedical Materials Research. Part A, vol. 87, no. 3, Dec. 2008, pp. 728–38. Epmc, doi:10.1002/jbm.a.31762.
Agarwal A, Jaye DL, Giegerman CM, Bellamkonda RV. Rational identification of a novel peptide for targeting nanocarriers to 9L glioma. Journal of biomedical materials research Part A. 2008 Dec;87(3):728–738.
Journal cover image

Published In

Journal of biomedical materials research. Part A

DOI

EISSN

1552-4965

ISSN

1549-3296

Publication Date

December 2008

Volume

87

Issue

3

Start / End Page

728 / 738

Related Subject Headings

  • Tumor Cells, Cultured
  • Rats, Inbred F344
  • Rats
  • Peptides
  • Nanostructures
  • Male
  • Liposomes
  • Gliosarcoma
  • Drug Carriers
  • Doxorubicin