Evaluation of a Fully Automated Research Prototype for the Immediate Identification of Microorganisms from Positive Blood Cultures under Clinical Conditions.


Journal Article

UNLABELLED:A clinical laboratory evaluation of an intrinsic fluorescence spectroscopy (IFS)-based identification system paired to a BacT/Alert Virtuo microbial detection system (bioMérieux, Inc., Durham, NC) was performed to assess the potential for fully automated identification of positive blood cultures. The prototype IFS system incorporates a novel method combining a simple microbial purification procedure with rapid in situ identification via spectroscopy. Results were available within 15 min of a bottle signaling positive and required no manual intervention. Among cultures positive for organisms contained within the database and producing acceptable spectra, 75 of 88 (85.2%) and 79 of 88 (89.8%) were correctly identified to the species and genus level, respectively. These results are similar to the performance of existing rapid methods. IMPORTANCE:A fully automated research platform was developed to identify microbial growth from positive blood cultures in <15 min. Because of the automated format, results can be generated during all shifts, with or without staffing, which in turn could promote more timely administration of target antimicrobial therapy.

Full Text

Cited Authors

  • Hyman, JM; Walsh, JD; Ronsick, C; Wilson, M; Hazen, KC; Borzhemskaya, L; Link, J; Clay, B; Ullery, M; Sanchez-Illan, M; Rothenberg, S; Robinson, R; van Belkum, A; Dunne, WM

Published Date

  • April 19, 2016

Published In

Volume / Issue

  • 7 / 2

Start / End Page

  • e00491 - e00416

PubMed ID

  • 27094332

Pubmed Central ID

  • 27094332

Electronic International Standard Serial Number (EISSN)

  • 2150-7511

International Standard Serial Number (ISSN)

  • 2150-7511

Digital Object Identifier (DOI)

  • 10.1128/mbio.00491-16


  • eng