A long non-coding RNA targets microRNA miR-34a to regulate colon cancer stem cell asymmetric division.
Journal Article (Journal Article)
The roles of long non-coding RNAs (lncRNAs) in regulating cancer and stem cells are being increasingly appreciated. Its diverse mechanisms provide the regulatory network with a bigger repertoire to increase complexity. Here we report a novel LncRNA, Lnc34a, that is enriched in colon cancer stem cells (CCSCs) and initiates asymmetric division by directly targeting the microRNA miR-34a to cause its spatial imbalance. Lnc34a recruits Dnmt3a via PHB2 and HDAC1 to methylate and deacetylate the miR-34a promoter simultaneously, hence epigenetically silencing miR-34a expression independent of its upstream regulator, p53. Lnc34a levels affect CCSC self-renewal and colorectal cancer (CRC) growth in xenograft models. Lnc34a is upregulated in late-stage CRCs, contributing to epigenetic miR-34a silencing and CRC proliferation. The fact that lncRNA targets microRNA highlights the regulatory complexity of non-coding RNAs (ncRNAs), which occupy the bulk of the genome.
Full Text
Duke Authors
Cited Authors
- Wang, L; Bu, P; Ai, Y; Srinivasan, T; Chen, HJ; Xiang, K; Lipkin, SM; Shen, X
Published Date
- April 14, 2016
Published In
Volume / Issue
- 5 /
PubMed ID
- 27077950
Pubmed Central ID
- 27077950
Electronic International Standard Serial Number (EISSN)
- 2050-084X
International Standard Serial Number (ISSN)
- 2050-084X
Digital Object Identifier (DOI)
- 10.7554/elife.14620
Language
- eng