Human prostate carcinoma cells express enzymatic activity that converts human plasminogen to the angiogenesis inhibitor, angiostatin.


Journal Article

Angiostatin is an inhibitor of angiogenesis and metastatic growth that is found in tumor-bearing animals and can be generated in vitro by the proteolytic cleavage of plasminogen. The mechanism by which angiostatin is produced in vivo has not been defined. We now demonstrate that human prostate carcinoma cell lines (PC-3, DU-145, and LN-CaP) express enzymatic activity that can generate bioactive angiostatin from purified human plasminogen or plasmin. Affinity purified PC-3-derived angiostatin inhibited human endothelial cell proliferation, basic fibroblast growth factor-induced migration, endothelial cell tube formation, and basic fibroblast growth factor-induced corneal angiogenesis. Studies with proteinase inhibitors demonstrated that a serine proteinase is necessary for angiostatin generation. These data indicate that bioactive angiostatin can be generated directly by human prostate cancer cells and that serine proteinase activity is necessary for angiostatin generation.

Full Text

Cited Authors

  • Gately, S; Twardowski, P; Stack, MS; Patrick, M; Boggio, L; Cundiff, DL; Schnaper, HW; Madison, L; Volpert, O; Bouck, N; Enghild, J; Kwaan, HC; Soff, GA

Published Date

  • November 1996

Published In

Volume / Issue

  • 56 / 21

Start / End Page

  • 4887 - 4890

PubMed ID

  • 8895739

Pubmed Central ID

  • 8895739

Electronic International Standard Serial Number (EISSN)

  • 1538-7445

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng