Impact of intestinal electrical stimulation on nutrient-induced GLP-1 secretion in vivo.

Published

Journal Article

BACKGROUND: Increases in L-cell release of GLP-1 are proposed to serve as a negative feedback signal for postprandial changes in gastric emptying and/or motility. Previous ex vivo data suggests that direct electrical stimulation (E-stim) of ileal segments stimulates secretion of GLP-1. This suggests potential feed-forward increases in GLP-1 driven by intestinal neuronal and/or motor activity. METHODS: To determine if E-stim could increase GLP-1 levels in an in vivo setting, we administered E-stim and nutrients to male Long- Evans rats (300-350 g) under general anesthesia. KEY RESULTS: Nutrient infusion into the duodenum or ileum significantly increased plasma GLP-1 levels, but E-stim applied to these locations did not (P < 0.05). However, the combination of E-stim and nutrient infusion, in either the ileum or duodenum, significantly increased plasma GLP-1 when compared to nutrient infusion alone (P < 0.05), and this effect was not blocked by either norepinephrine or atropine. To test the impact of intestinal motor activity, the effect of extra-luminal mechanical stimulation (M-stim) on GLP-1 levels was assessed. In the duodenum, but not the ileum, M-stim plus nutrient infusion significantly increased GLP-1 over nutrient infusion or M-stim alone (P < 0.05). CONCLUSIONS & INFERENCES: Thus, both E- and M-stim of the duodenum, but only E-stim of the ileum augmented nutrient-stimulated GLP-1 release. These data demonstrate that factors beyond enteral nutrients could contribute to the regulation of GLP-1 secretion.

Full Text

Duke Authors

Cited Authors

  • Sandoval, D; Dunki-Jacobs, A; Sorrell, J; Seeley, RJ; D'Alessio, DD

Published Date

  • August 2013

Published In

Volume / Issue

  • 25 / 8

Start / End Page

  • 700 - 705

PubMed ID

  • 23663526

Pubmed Central ID

  • 23663526

Electronic International Standard Serial Number (EISSN)

  • 1365-2982

Digital Object Identifier (DOI)

  • 10.1111/nmo.12152

Language

  • eng

Conference Location

  • England