In Vivo Selection Against Human Colorectal Cancer Xenografts Identifies an Aptamer That Targets RNA Helicase Protein DHX9.

Journal Article (Journal Article)

The ability to selectively target disease-related tissues with molecules is critical to the design of effective therapeutic and diagnostic reagents. Recognizing the differences between the in vivo environment and in vitro conditions, we employed an in vivo selection strategy to identify RNA aptamers (targeting motifs) that could localize to tumor in situ. One of the selected molecules is an aptamer that binds to the protein DHX9, an RNA helicase that is known to be upregulated in colorectal cancer. Upon systemic administration, the aptamer preferentially localized to the nucleus of cancer cells in vivo and thus has the potential to be used for targeted delivery.

Full Text

Duke Authors

Cited Authors

  • Mi, J; Ray, P; Liu, J; Kuan, C-T; Xu, J; Hsu, D; Sullenger, BA; White, RR; Clary, BM

Published Date

  • April 26, 2016

Published In

Volume / Issue

  • 5 / 4

Start / End Page

  • e315 -

PubMed ID

  • 27115840

Pubmed Central ID

  • PMC5014527

International Standard Serial Number (ISSN)

  • 2162-2531

Digital Object Identifier (DOI)

  • 10.1038/mtna.2016.27

Language

  • eng

Conference Location

  • United States