STAT6-independent production of IL-4 by mast cells.

Published

Journal Article

The acquisition of an IL-4-producing phenotype in Th2 cells requires IL-4 signaling through the STAT6 pathway during T cell differentiation. In this study we demonstrate that, unlike in naive T cells, IL-4 is not necessary for the development of an IL-4-producing phenotype in mast cells. Bone marrow-derived mast cell precursors from STAT6-/- mice can differentiate into mature cells that express IL-4 levels comparable to those of wild-type mast cells. In differentiated mast cells, activation in the presence of neutralizing anti-IL-4 antibodies or mutation of the consensus STAT6 sites does not diminish IL-4 promoter activity, indicating that IL-4 is not required for active transcription. Taken together, these data suggest that mast cell IL-4 production is not STAT6 dependent, providing evidence that these cells could generate IL-4 needed for the initiation and amplification of an effective Th2 immune response.

Full Text

Duke Authors

Cited Authors

  • Sherman, MA; Secor, VH; Lee, SK; Lopez, RD; Brown, MA

Published Date

  • April 1999

Published In

Volume / Issue

  • 29 / 4

Start / End Page

  • 1235 - 1242

PubMed ID

  • 10229091

Pubmed Central ID

  • 10229091

Electronic International Standard Serial Number (EISSN)

  • 1521-4141

International Standard Serial Number (ISSN)

  • 0014-2980

Digital Object Identifier (DOI)

  • 10.1002/(sici)1521-4141(199904)29:04<1235::aid-immu1235>3.0.co;2-0

Language

  • eng