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STAT6-independent production of IL-4 by mast cells.

Publication ,  Journal Article
Sherman, MA; Secor, VH; Lee, SK; Lopez, RD; Brown, MA
Published in: Eur J Immunol
April 1999

The acquisition of an IL-4-producing phenotype in Th2 cells requires IL-4 signaling through the STAT6 pathway during T cell differentiation. In this study we demonstrate that, unlike in naive T cells, IL-4 is not necessary for the development of an IL-4-producing phenotype in mast cells. Bone marrow-derived mast cell precursors from STAT6-/- mice can differentiate into mature cells that express IL-4 levels comparable to those of wild-type mast cells. In differentiated mast cells, activation in the presence of neutralizing anti-IL-4 antibodies or mutation of the consensus STAT6 sites does not diminish IL-4 promoter activity, indicating that IL-4 is not required for active transcription. Taken together, these data suggest that mast cell IL-4 production is not STAT6 dependent, providing evidence that these cells could generate IL-4 needed for the initiation and amplification of an effective Th2 immune response.

Duke Scholars

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

April 1999

Volume

29

Issue

4

Start / End Page

1235 / 1242

Location

Germany

Related Subject Headings

  • Trans-Activators
  • STAT6 Transcription Factor
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Mice, Inbred C57BL
  • Mice
  • Mast Cells
  • Interleukin-4
  • Immunology
  • Cells, Cultured
 

Citation

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ICMJE
MLA
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Sherman, M. A., Secor, V. H., Lee, S. K., Lopez, R. D., & Brown, M. A. (1999). STAT6-independent production of IL-4 by mast cells. Eur J Immunol, 29(4), 1235–1242. https://doi.org/10.1002/(SICI)1521-4141(199904)29:04<1235::AID-IMMU1235>3.0.CO;2-0
Sherman, M. A., V. H. Secor, S. K. Lee, R. D. Lopez, and M. A. Brown. “STAT6-independent production of IL-4 by mast cells.Eur J Immunol 29, no. 4 (April 1999): 1235–42. https://doi.org/10.1002/(SICI)1521-4141(199904)29:04<1235::AID-IMMU1235>3.0.CO;2-0.
Sherman MA, Secor VH, Lee SK, Lopez RD, Brown MA. STAT6-independent production of IL-4 by mast cells. Eur J Immunol. 1999 Apr;29(4):1235–42.
Sherman, M. A., et al. “STAT6-independent production of IL-4 by mast cells.Eur J Immunol, vol. 29, no. 4, Apr. 1999, pp. 1235–42. Pubmed, doi:10.1002/(SICI)1521-4141(199904)29:04<1235::AID-IMMU1235>3.0.CO;2-0.
Sherman MA, Secor VH, Lee SK, Lopez RD, Brown MA. STAT6-independent production of IL-4 by mast cells. Eur J Immunol. 1999 Apr;29(4):1235–1242.
Journal cover image

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

April 1999

Volume

29

Issue

4

Start / End Page

1235 / 1242

Location

Germany

Related Subject Headings

  • Trans-Activators
  • STAT6 Transcription Factor
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Mice, Inbred C57BL
  • Mice
  • Mast Cells
  • Interleukin-4
  • Immunology
  • Cells, Cultured