An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.

Journal Article

Cellular organelles provide opportunities to relate biological mechanisms to disease. Here we use affinity proteomics, genetics and cell biology to interrogate cilia: poorly understood organelles, where defects cause genetic diseases. Two hundred and seventeen tagged human ciliary proteins create a final landscape of 1,319 proteins, 4,905 interactions and 52 complexes. Reverse tagging, repetition of purifications and statistical analyses, produce a high-resolution network that reveals organelle-specific interactions and complexes not apparent in larger studies, and links vesicle transport, the cytoskeleton, signalling and ubiquitination to ciliary signalling and proteostasis. We observe sub-complexes in exocyst and intraflagellar transport complexes, which we validate biochemically, and by probing structurally predicted, disruptive, genetic variants from ciliary disease patients. The landscape suggests other genetic diseases could be ciliary including 3M syndrome. We show that 3M genes are involved in ciliogenesis, and that patient fibroblasts lack cilia. Overall, this organelle-specific targeting strategy shows considerable promise for Systems Medicine.

Full Text

Duke Authors

Cited Authors

  • Boldt, K; van Reeuwijk, J; Lu, Q; Koutroumpas, K; Nguyen, T-MT; Texier, Y; van Beersum, SEC; Horn, N; Willer, JR; Mans, DA; Dougherty, G; Lamers, IJC; Coene, KLM; Arts, HH; Betts, MJ; Beyer, T; Bolat, E; Gloeckner, CJ; Haidari, K; Hetterschijt, L; Iaconis, D; Jenkins, D; Klose, F; Knapp, B; Latour, B; Letteboer, SJF; Marcelis, CL; Mitic, D; Morleo, M; Oud, MM; Riemersma, M; Rix, S; Terhal, PA; Toedt, G; van Dam, TJP; de Vrieze, E; Wissinger, Y; Wu, KM; Apic, G; Beales, PL; Blacque, OE et al.

Published Date

  • May 13, 2016

Published In

Volume / Issue

  • 7 /

Start / End Page

  • 11491 -

PubMed ID

  • 27173435

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms11491

Language

  • eng