Imaging whole-brain cytoarchitecture of mouse with MRI-based quantitative susceptibility mapping.

Journal Article (Journal Article)

The proper microstructural arrangement of complex neural structures is essential for establishing the functional circuitry of the brain. We present an MRI method to resolve tissue microstructure and infer brain cytoarchitecture by mapping the magnetic susceptibility in the brain at high resolution. This is possible because of the heterogeneous magnetic susceptibility created by varying concentrations of lipids, proteins and irons from the cell membrane to cytoplasm. We demonstrate magnetic susceptibility maps at a nominal resolution of 10-μm isotropic, approaching the average cell size of a mouse brain. The maps reveal many detailed structures including the retina cell layers, olfactory sensory neurons, barrel cortex, cortical layers, axonal fibers in white and gray matter. Olfactory glomerulus density is calculated and structural connectivity is traced in the optic nerve, striatal neurons, and brainstem nerves. The method is robust and can be readily applied on MRI scanners at or above 7T.

Full Text

Duke Authors

Cited Authors

  • Wei, H; Xie, L; Dibb, R; Li, W; Decker, K; Zhang, Y; Johnson, GA; Liu, C

Published Date

  • August 15, 2016

Published In

Volume / Issue

  • 137 /

Start / End Page

  • 107 - 115

PubMed ID

  • 27181764

Pubmed Central ID

  • PMC5201162

Electronic International Standard Serial Number (EISSN)

  • 1095-9572

Digital Object Identifier (DOI)

  • 10.1016/j.neuroimage.2016.05.033


  • eng

Conference Location

  • United States