Skip to main content
Journal cover image

The effect of irrigation solution at different temperatures on articular cartilage metabolism.

Publication ,  Journal Article
Kocaoglu, B; Martin, J; Wolf, B; Karahan, M; Amendola, A
Published in: Arthroscopy
April 2011

PURPOSE: The purpose of this study was to determine the effects of saline solution at different temperatures on the metabolism of chondrocytes. METHODS: Porcine osteochondral explants were precultured under laboratory conditions. The cartilage explants were placed in saline solution. Twenty-four explants were randomly divided into 4 groups. Explants were immersed at 4°C group I, at room temperature (24°C) in group II, at normal knee temperature (32°C) in group III, and at near-core body temperature (37°C) in group IV. All specimens were immersed for 2 hours. Lactate and proteoglycan production and RNA yield analysis were performed to evaluate the changes in cartilage metabolism at different temperatures. RESULTS: Explants immersed in cold (4°C) saline solution showed the significantly lowest RNA yields, lactate production, and proteoglycan content. Explants immersed in cold solutions (4°C and 24°C) showed significantly lower RNA yields, lower lactate production, and lower proteoglycan content than explants in warmer solution groups (32°C and 37°C). CONCLUSION: The findings of this study suggest that short-term exposures to cold including room temperature may have markedly detrimental effects on chondrocyte function. Our findings also indicate that exposures to cold saline solution suppress chondrocyte metabolism and RNA synthesis. CLINICAL RELEVANCE: Using warmer irrigation solution that is closer to body temperature is more physiologic and causes less ultrastructural damage than colder solution.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Arthroscopy

DOI

EISSN

1526-3231

Publication Date

April 2011

Volume

27

Issue

4

Start / End Page

526 / 531

Location

United States

Related Subject Headings

  • Therapeutic Irrigation
  • Temperature
  • Swine
  • Sus scrofa
  • Sodium Chloride
  • Random Allocation
  • RNA, Messenger
  • Proteoglycans
  • Polymerase Chain Reaction
  • Orthopedics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kocaoglu, B., Martin, J., Wolf, B., Karahan, M., & Amendola, A. (2011). The effect of irrigation solution at different temperatures on articular cartilage metabolism. Arthroscopy, 27(4), 526–531. https://doi.org/10.1016/j.arthro.2010.10.019
Kocaoglu, Baris, James Martin, Brian Wolf, Mustafa Karahan, and Annunziato Amendola. “The effect of irrigation solution at different temperatures on articular cartilage metabolism.Arthroscopy 27, no. 4 (April 2011): 526–31. https://doi.org/10.1016/j.arthro.2010.10.019.
Kocaoglu B, Martin J, Wolf B, Karahan M, Amendola A. The effect of irrigation solution at different temperatures on articular cartilage metabolism. Arthroscopy. 2011 Apr;27(4):526–31.
Kocaoglu, Baris, et al. “The effect of irrigation solution at different temperatures on articular cartilage metabolism.Arthroscopy, vol. 27, no. 4, Apr. 2011, pp. 526–31. Pubmed, doi:10.1016/j.arthro.2010.10.019.
Kocaoglu B, Martin J, Wolf B, Karahan M, Amendola A. The effect of irrigation solution at different temperatures on articular cartilage metabolism. Arthroscopy. 2011 Apr;27(4):526–531.
Journal cover image

Published In

Arthroscopy

DOI

EISSN

1526-3231

Publication Date

April 2011

Volume

27

Issue

4

Start / End Page

526 / 531

Location

United States

Related Subject Headings

  • Therapeutic Irrigation
  • Temperature
  • Swine
  • Sus scrofa
  • Sodium Chloride
  • Random Allocation
  • RNA, Messenger
  • Proteoglycans
  • Polymerase Chain Reaction
  • Orthopedics