Skip to main content

An Automated High-throughput Array Microscope for Cancer Cell Mechanics.

Publication ,  Journal Article
Cribb, JA; Osborne, LD; Beicker, K; Psioda, M; Chen, J; O'Brien, ET; Taylor Ii, RM; Vicci, L; Hsiao, JP-L; Shao, C; Falvo, M; Ibrahim, JG ...
Published in: Sci Rep
June 6, 2016

Changes in cellular mechanical properties correlate with the progression of metastatic cancer along the epithelial-to-mesenchymal transition (EMT). Few high-throughput methodologies exist that measure cell compliance, which can be used to understand the impact of genetic alterations or to screen the efficacy of chemotherapeutic agents. We have developed a novel array high-throughput microscope (AHTM) system that combines the convenience of the standard 96-well plate with the ability to image cultured cells and membrane-bound microbeads in twelve independently-focusing channels simultaneously, visiting all wells in eight steps. We use the AHTM and passive bead rheology techniques to determine the relative compliance of human pancreatic ductal epithelial (HPDE) cells, h-TERT transformed HPDE cells (HPNE), and four gain-of-function constructs related to EMT. The AHTM found HPNE, H-ras, Myr-AKT, and Bcl2 transfected cells more compliant relative to controls, consistent with parallel tests using atomic force microscopy and invasion assays, proving the AHTM capable of screening for changes in mechanical phenotype.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

June 6, 2016

Volume

6

Start / End Page

27371

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Pancreatic Neoplasms
  • Microscopy
  • Humans
  • High-Throughput Nucleotide Sequencing
  • Automation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cribb, J. A., Osborne, L. D., Beicker, K., Psioda, M., Chen, J., O’Brien, E. T., … Superfine, R. (2016). An Automated High-throughput Array Microscope for Cancer Cell Mechanics. Sci Rep, 6, 27371. https://doi.org/10.1038/srep27371
Cribb, Jeremy A., Lukas D. Osborne, Kellie Beicker, Matthew Psioda, Jian Chen, E Timothy O’Brien, Russell M. Taylor Ii, et al. “An Automated High-throughput Array Microscope for Cancer Cell Mechanics.Sci Rep 6 (June 6, 2016): 27371. https://doi.org/10.1038/srep27371.
Cribb JA, Osborne LD, Beicker K, Psioda M, Chen J, O’Brien ET, et al. An Automated High-throughput Array Microscope for Cancer Cell Mechanics. Sci Rep. 2016 Jun 6;6:27371.
Cribb, Jeremy A., et al. “An Automated High-throughput Array Microscope for Cancer Cell Mechanics.Sci Rep, vol. 6, June 2016, p. 27371. Pubmed, doi:10.1038/srep27371.
Cribb JA, Osborne LD, Beicker K, Psioda M, Chen J, O’Brien ET, Taylor Ii RM, Vicci L, Hsiao JP-L, Shao C, Falvo M, Ibrahim JG, Wood KC, Blobe GC, Superfine R. An Automated High-throughput Array Microscope for Cancer Cell Mechanics. Sci Rep. 2016 Jun 6;6:27371.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

June 6, 2016

Volume

6

Start / End Page

27371

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Pancreatic Neoplasms
  • Microscopy
  • Humans
  • High-Throughput Nucleotide Sequencing
  • Automation