Imaging Polarization in Budding Yeast.

Journal Article (Journal Article)

We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected aspects of polarization including transient intermediates with more than one polarity cluster, oscillatory clustering of polarity factors, and mobile "wandering" polarity sites.

Full Text

Duke Authors

Cited Authors

  • McClure, AW; Wu, C-F; Johnson, SA; Lew, DJ

Published Date

  • 2016

Published In

Volume / Issue

  • 1407 /

Start / End Page

  • 13 - 23

PubMed ID

  • 27271891

Pubmed Central ID

  • PMC5934585

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-3480-5_2

Language

  • eng

Conference Location

  • United States