Porous biocompatible three-dimensional scaffolds of cellulose microfiber/gelatin composites for cell culture.

Published

Journal Article

Physiological tissues, including brain and other organs, have three-dimensional (3-D) aspects that need to be supported to model them in vitro. Here we report the use of cellulose microfibers combined with cross-linked gelatin to make biocompatible porous microscaffolds for the sustained growth of brain cell and human mesenchymal stem cells (hMSCs) in 3-D structure. Live imaging using confocal microscopy indicated that 3-D microscaffolds composed of gelatin or cellulose fiber/gelatin both supported brain cell adhesion and growth for 16days in vitro. Cellulose microfiber/gelatin composites containing up to 75% cellulose fibers can withstand a higher mechanical load than gelatin alone, and composites also provided linear pathways along which brain cells could grow compared to more clumped cell growth in gelatin alone. Therefore, the bulk cellulose microfiber provides a novel skeleton in this new scaffold material. Cellulose fiber/gelatin scaffold supported hMSCs growth and extracellular matrix formation. hMSCs osteogenic and adipogenic assays indicated that hMSCs cultured in cellulose fiber/gelatin composite preserved the multilineage differentiation potential. As natural, biocompatible components, the combination of gelatin and cellulose microfibers, fabricated into 3-D matrices, may therefore provide optimal porosity and tensile strength for long-term maintenance and observation of cells.

Full Text

Duke Authors

Cited Authors

  • Xing, Q; Zhao, F; Chen, S; McNamara, J; Decoster, MA; Lvov, YM

Published Date

  • June 2010

Published In

Volume / Issue

  • 6 / 6

Start / End Page

  • 2132 - 2139

PubMed ID

  • 20035906

Pubmed Central ID

  • 20035906

Electronic International Standard Serial Number (EISSN)

  • 1878-7568

Digital Object Identifier (DOI)

  • 10.1016/j.actbio.2009.12.036

Language

  • eng

Conference Location

  • England