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Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development.

Publication ,  Journal Article
Macaulay, LJ; Chernick, M; Chen, A; Hinton, DE; Bailey, JM; Kullman, SW; Levin, ED; Stapleton, HM
Published in: Environ Toxicol Chem
January 2017

Polybrominated diphenyl ethers (PBDEs) and their metabolites (e.g., hydroxylated BDEs [OH-BDEs]) are contaminants frequently detected together in human tissues and are structurally similar to thyroid hormones. Thyroid hormones partially mediate metamorphic transitions between life stages in zebrafish, making this a critical developmental window that may be vulnerable to chemicals disrupting thyroid signaling. In the present study, zebrafish were exposed to 6-OH-BDE-47 (30 nM; 15 μg/L) alone, or to a low-dose (30 μg/L) or high-dose (600 μg/L) mixture of PentaBDEs, 6-OH-BDE-47 (0.5-6 μg/L), and 2,4,6-tribromophenol (5-100 μg/L) during juvenile development (9-23 d postfertilization) and evaluated for developmental endpoints mediated by thyroid hormone signaling. Fish were sampled at 3 time points and examined for developmental and skeletal morphology, apical thyroid and skeletal gene markers, and modifications in swimming behavior (as adults). Exposure to the high-dose mixture resulted in >85% mortality within 1 wk of exposure, despite being below reported acute toxicity thresholds for individual congeners. The low-dose mixture and 6-OH-BDE-47 groups exhibited reductions in body length and delayed maturation, specifically relating to swim bladder, fin, and pigmentation development. Reduced skeletal ossification was also observed in 6-OH-BDE-47-treated fish. Assessment of thyroid and osteochondral gene regulatory networks demonstrated significantly increased expression of genes that regulate skeletal development and thyroid hormones. Overall, these results indicate that exposures to PBDE/OH-BDE mixtures adversely impact zebrafish maturation during metamorphosis. Environ Toxicol Chem 2017;36:36-48. © 2016 SETAC.

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Published In

Environ Toxicol Chem

DOI

EISSN

1552-8618

Publication Date

January 2017

Volume

36

Issue

1

Start / End Page

36 / 48

Location

United States

Related Subject Headings

  • Zebrafish
  • Thyroid Hormones
  • Thyroid Gland
  • Polybrominated Biphenyls
  • Osteogenesis
  • Humans
  • Halogenated Diphenyl Ethers
  • Gene Expression
  • Environmental Sciences
  • Dose-Response Relationship, Drug
 

Citation

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Macaulay, L. J., Chernick, M., Chen, A., Hinton, D. E., Bailey, J. M., Kullman, S. W., … Stapleton, H. M. (2017). Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development. Environ Toxicol Chem, 36(1), 36–48. https://doi.org/10.1002/etc.3535
Macaulay, Laura J., Melissa Chernick, Albert Chen, David E. Hinton, Jordan M. Bailey, Seth W. Kullman, Edward D. Levin, and Heather M. Stapleton. “Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development.Environ Toxicol Chem 36, no. 1 (January 2017): 36–48. https://doi.org/10.1002/etc.3535.
Macaulay LJ, Chernick M, Chen A, Hinton DE, Bailey JM, Kullman SW, et al. Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development. Environ Toxicol Chem. 2017 Jan;36(1):36–48.
Macaulay, Laura J., et al. “Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development.Environ Toxicol Chem, vol. 36, no. 1, Jan. 2017, pp. 36–48. Pubmed, doi:10.1002/etc.3535.
Macaulay LJ, Chernick M, Chen A, Hinton DE, Bailey JM, Kullman SW, Levin ED, Stapleton HM. Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development. Environ Toxicol Chem. 2017 Jan;36(1):36–48.
Journal cover image

Published In

Environ Toxicol Chem

DOI

EISSN

1552-8618

Publication Date

January 2017

Volume

36

Issue

1

Start / End Page

36 / 48

Location

United States

Related Subject Headings

  • Zebrafish
  • Thyroid Hormones
  • Thyroid Gland
  • Polybrominated Biphenyls
  • Osteogenesis
  • Humans
  • Halogenated Diphenyl Ethers
  • Gene Expression
  • Environmental Sciences
  • Dose-Response Relationship, Drug