Dynamic protein coronas revealed as a modulator of silver nanoparticle sulphidation in vitro.

Published

Journal Article

Proteins adsorbing at nanoparticles have been proposed as critical toxicity mediators and are included in ongoing efforts to develop predictive tools for safety assessment. Strongly attached proteins can be isolated, identified and correlated to changes in nanoparticle state, cellular association or toxicity. Weakly attached, rapidly exchanging proteins are also present at nanoparticles, but are difficult to isolate and have hardly been examined. Here we study rapidly exchanging proteins and show for the first time that they have a strong modulatory effect on the biotransformation of silver nanoparticles. Released silver ions, known for their role in particle toxicity, are found to be trapped as silver sulphide nanocrystals within the protein corona at silver nanoparticles in serum-containing cell culture media. The strongly attached corona acts as a site for sulphidation, while the weakly attached proteins reduce nanocrystal formation in a serum-concentration-dependent manner. Sulphidation results in decreased toxicity of Ag NPs.

Full Text

Cited Authors

  • Miclăuş, T; Beer, C; Chevallier, J; Scavenius, C; Bochenkov, VE; Enghild, JJ; Sutherland, DS

Published Date

  • June 9, 2016

Published In

Volume / Issue

  • 7 /

Start / End Page

  • 11770 -

PubMed ID

  • 27278102

Pubmed Central ID

  • 27278102

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms11770

Language

  • eng