Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney.
Published
Journal Article
To identify kidney glutathione S-transferase (GST) isoenzyme, which does not bind to glutathione affinity column, biochemical characterization was performed by using an array of substrates and by measuring sensitivity to inhibitors. Immunological characterization was done by immunoblotting. Affinity flow-through GST exhibited activity towards 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole and cumene hydroperoxide, typical class alpha substrates and high sensitivity towards hematin, an alpha class inhibitor. It cross-reacted with antibodies against alpha class GST. Affinity flow-through GST in human kidney is an alpha class member.
Full Text
Duke Authors
Cited Authors
- Simic, T; Pljesa-Ercegovac, M; Savic-Radojevic, A; Hadziahmetovic, M; Mimic-Oka, J
Published Date
- June 2006
Published In
Volume / Issue
- 30 / 4
Start / End Page
- 495 - 498
PubMed ID
- 16773246
Pubmed Central ID
- 16773246
International Standard Serial Number (ISSN)
- 0939-4451
Digital Object Identifier (DOI)
- 10.1007/s00726-006-0329-7
Language
- eng
Conference Location
- Austria