Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney.

Journal Article (Journal Article)

To identify kidney glutathione S-transferase (GST) isoenzyme, which does not bind to glutathione affinity column, biochemical characterization was performed by using an array of substrates and by measuring sensitivity to inhibitors. Immunological characterization was done by immunoblotting. Affinity flow-through GST exhibited activity towards 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole and cumene hydroperoxide, typical class alpha substrates and high sensitivity towards hematin, an alpha class inhibitor. It cross-reacted with antibodies against alpha class GST. Affinity flow-through GST in human kidney is an alpha class member.

Full Text

Duke Authors

Cited Authors

  • Simic, T; Pljesa-Ercegovac, M; Savic-Radojevic, A; Hadziahmetovic, M; Mimic-Oka, J

Published Date

  • June 2006

Published In

Volume / Issue

  • 30 / 4

Start / End Page

  • 495 - 498

PubMed ID

  • 16773246

International Standard Serial Number (ISSN)

  • 0939-4451

Digital Object Identifier (DOI)

  • 10.1007/s00726-006-0329-7


  • eng

Conference Location

  • Austria